Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,512,887	G→A	V27I (GTC→ATC)	gltS →	glutamate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,512,887	0	G	A	100.0%	284.1 / NA	87	V27I (GTC→ATC)	gltS	glutamate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (53/34);  total (53/34)

TCGATACTTTAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCGTCTCCTTTTTGAAGAAATACACCATACCGGAACCTGTTGCGGGTGGTTTGTTGG  >  minE/2512819‑2512941                                                                     |                                                       tcGATACTTTAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCAtc                                                      >  1:1153393/1‑71 (MQ=255) tcGATACTTTAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCAtc                                                      >  1:2648300/1‑71 (MQ=255) tcGATACTTTAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCAtc                                                      >  1:4066436/1‑71 (MQ=255) tcGATACTTTAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCAtc                                                      >  1:4051170/1‑71 (MQ=255) tcGATACTTTAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCAtc                                                      >  1:4048229/1‑71 (MQ=255) tcGATACTTTAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCAtc                                                      >  1:3132194/1‑71 (MQ=255) tcGATACTTTAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCAtc                                                      >  1:2056411/1‑71 (MQ=255) tcGATACTTTAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCAATTCCAtc                                                      >  1:2820924/1‑71 (MQ=255)     tACTTTAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  <  1:2868521/71‑1 (MQ=255)         ttggCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCttttt                                              >  1:840475/4‑71 (MQ=255)         ttAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCttttt                                              >  1:3489119/1‑71 (MQ=255)         ttAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCttttt                                              >  1:2885241/1‑71 (MQ=255)         ttAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCttttt                                              >  1:209071/1‑71 (MQ=255)         ttAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCttttt                                              >  1:331735/1‑71 (MQ=255)         ttAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCttttt                                              >  1:3341704/1‑71 (MQ=255)         ttAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCttttt                                              >  1:170610/1‑71 (MQ=255)         ttAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCttttt                                              >  1:1418106/1‑71 (MQ=255)         ttAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCttttt                                              >  1:3662643/1‑71 (MQ=255)         ttAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCttttt                                              >  1:3642945/1‑71 (MQ=255)         ttAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCttttt                                              >  1:3631761/1‑71 (MQ=255)         ttAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCttttt                                              >  1:1659163/1‑71 (MQ=255)              aaCGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTgaaga                                         <  1:3135504/71‑1 (MQ=255)              aaCGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTgaaga                                         <  1:3627299/71‑1 (MQ=255)              aaCGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTgaaga                                         <  1:3411374/71‑1 (MQ=255)              aaCGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTgaaga                                         <  1:2960017/71‑1 (MQ=255)              aaCGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTgaaga                                         <  1:2404131/71‑1 (MQ=255)              aaCGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTgaaga                                         <  1:2409023/71‑1 (MQ=255)              aaCGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTgaaga                                         <  1:3764905/71‑1 (MQ=255)              aaCGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTgaaga                                         <  1:3867599/71‑1 (MQ=255)              aaCGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTgaaga                                         <  1:2788568/71‑1 (MQ=255)              aaCGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTgaaga                                         <  1:731308/71‑1 (MQ=255)              aaCGCTTGTTGCAGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTgaaga                                         <  1:2633761/71‑1 (MQ=255)               aCGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTgaaga                                         <  1:1092109/70‑1 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTcc                                                   >  1:3249396/1‑59 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:4336571/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:3723399/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:1396927/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:3943283/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:4092595/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:4177473/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:4328145/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:3144744/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:2064966/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:4377776/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:550827/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:2902271/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:2885549/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:744771/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:2539633/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:1086023/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:890238/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:2568994/1‑60 (MQ=255)                cGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTCGTCCATTCCAtctc                                                    >  1:3127265/1‑58 (MQ=255)                cGCTTGATGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCt                                                  >  1:562695/1‑60 (MQ=255)                 tcTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGaaa                                       >  1:4216861/2‑70 (MQ=255)                 gCTTGTTGCCGCAACGCTGCCGTTGCTGCTCGGGCGTATGTTGGTCCATTCCATCTCCTTTTTGAAGaaa                                       >  1:1996425/1‑70 (MQ=255)                 gCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGaaa                                       >  1:1423059/1‑70 (MQ=255)                 gCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGaaa                                       >  1:1293106/1‑70 (MQ=255)                 gCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGaaa                                       >  1:1378083/1‑70 (MQ=255)                 gCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGaaa                                       >  1:1396537/1‑70 (MQ=255)                 gCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGaaa                                       >  1:1499726/1‑70 (MQ=255)                 gCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGaaa                                       >  1:3192597/1‑70 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:967020/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:2632244/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:885382/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:2543454/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:796515/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:2405857/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:2934839/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:3019384/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:2243484/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:3302394/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:1974629/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:1316421/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:1321622/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:1905320/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:3990474/71‑1 (MQ=255)                  cTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:3851012/71‑1 (MQ=255)                             aaCGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATa                                     <  1:3227730/60‑1 (MQ=255)                              aCGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATACACCATACCgg                          >  1:868574/1‑70 (MQ=255)                              aCGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATACACCATACCgg                          >  1:1464749/1‑70 (MQ=255)                              aCGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATACACCATACCg                           >  1:748428/1‑69 (MQ=255)                                                     tAAGTTGGTCCATTCCATCTCCTTTTTGAAGAAATACACCATACCGGAACCTGTTGCGGGTGGTTTGTtgg  >  1:2702481/1‑71 (MQ=255)                                                        gTTGGTCCATTCCATCTCCTTTTTGAAGAAATACACCATACCGGAACCTGTTGCGggtggt         <  1:2313022/61‑1 (MQ=255)                                                        gTTGGTCCATTCCATCTCCTTTTTGAAGAAATACACCATACCGGAACCTGTTGCGggtggt         <  1:2513691/61‑1 (MQ=255)                                                        gTTGGTCCATTCCATCTCCTTTTTGAAGAAATACACCATACCGGAACCTGTTGCGggtggt         <  1:1706733/61‑1 (MQ=255)                                                        gTTGGTCCATTCCATCTCCTTTTTGAAGAAATACACCATACCGGAACCTGTTGCGggtggt         <  1:1688701/61‑1 (MQ=255)                                                                     |                                                       TCGATACTTTAGCAACGCTTGTTGCCGCAACGCTGACGTTGCTGCTCGGGCGTAAGTTGGTCCATTCCGTCTCCTTTTTGAAGAAATACACCATACCGGAACCTGTTGCGGGTGGTTTGTTGG  >  minE/2512819‑2512941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑