Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 2,516,656 C→T A102T (GCT→ACT)  trmH ← tRNA (Guanosine‑2'‑O‑)‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,516,6560CT100.0% 338.2 / NA 103A102T (GCT→ACT) trmHtRNA (Guanosine‑2'‑O‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/80);  total (23/80)

Only 100 of 103 total aligned reads displayed.

CCGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATG  >  minE/2516590‑2516707
                                                                  |                                                   
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:4369154/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:2718320/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:2452548/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:2910522/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:4327944/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:303004/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:4373883/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:1753051/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:3274420/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:164528/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:1489289/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:3219975/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:844452/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:1121755/71‑1 (MQ=255)
ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGACTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                 <  1:1554987/71‑1 (MQ=255)
      tctcCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGa                                            <  1:4233458/70‑1 (MQ=255)
      tctcCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGa                                            <  1:1651980/70‑1 (MQ=255)
      tctcCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGa                                            <  1:520327/70‑1 (MQ=255)
      tctcCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGa                                            <  1:2873813/70‑1 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGaaa                                          <  1:812516/68‑1 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGaaa                                          <  1:3179886/68‑1 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGaaa                                          <  1:3521574/68‑1 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGaaa                                          <  1:3453717/68‑1 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGaaa                                          <  1:2765742/68‑1 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGAt                                       >  1:3361121/1‑71 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGAt                                       >  1:3988082/1‑71 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGAt                                       >  1:2733792/1‑71 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGAt                                       >  1:1003530/1‑71 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGAt                                       >  1:2185520/1‑71 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGAt                                       >  1:2987965/1‑71 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGAt                                       >  1:2007953/1‑71 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGAt                                       >  1:3328099/1‑71 (MQ=255)
          cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGAt                                       >  1:735483/1‑71 (MQ=255)
          cTGTCACATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGa                                        >  1:3009389/1‑70 (MQ=255)
             tCCCATCAAAATGCACGTCGGGCGAGTGTAATCAATTTGGCGGAAATCGACAGTGTTa                                                 <  1:428894/58‑1 (MQ=255)
                    aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTg                                 >  1:637619/1‑67 (MQ=255)
                    aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc                                >  1:516576/1‑68 (MQ=255)
                    aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc                                >  1:4195254/1‑68 (MQ=255)
                    aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc                                >  1:2910636/1‑68 (MQ=255)
                    aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc                                >  1:4148116/1‑68 (MQ=255)
                    aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc                                >  1:2904277/1‑68 (MQ=255)
                    aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc                                >  1:493709/1‑68 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCTCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:3198787/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAGATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:2848152/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:2706852/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:1167666/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:3171573/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:3165773/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:1349688/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:1711078/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:1793274/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:1893776/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:1988668/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:4241932/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:217075/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:2279876/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:404641/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:2431534/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:2799990/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:3393629/70‑1 (MQ=255)
                          cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg                       <  1:271513/71‑1 (MQ=255)
                           aGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:3643202/69‑1 (MQ=255)
                           aGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:984684/69‑1 (MQ=255)
                           aGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg                       <  1:2386233/70‑1 (MQ=255)
                               cGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:4067349/65‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:1397251/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:1777738/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:1925978/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:4250358/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:77795/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:118896/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:1132941/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:4119362/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:4114299/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:2344167/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:2382214/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:2451981/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:350429/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:3014226/71‑1 (MQ=255)
                                  gCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCt               <  1:311362/71‑1 (MQ=255)
                                       tgtAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCt          <  1:4041775/71‑1 (MQ=255)
                                       tgtAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCt          <  1:3647558/71‑1 (MQ=255)
                                       tgtAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCt          <  1:2226270/71‑1 (MQ=255)
                                       tgtAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCt          <  1:3397491/71‑1 (MQ=255)
                                       tgtAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCt          <  1:2107594/71‑1 (MQ=255)
                                        gtAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATTGGTTGCCAGAATCTGCATGCCCTGGCCt          <  1:2969728/70‑1 (MQ=255)
                                         tAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg                       <  1:2494068/56‑1 (MQ=255)
                                         tAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg                       <  1:2588391/56‑1 (MQ=255)
                                         tAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg                       <  1:1215352/56‑1 (MQ=255)
                                             cAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt        >  1:1280595/1‑67 (MQ=255)
                                             cAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt        >  1:101103/1‑67 (MQ=255)
                                             cAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt        >  1:213233/1‑67 (MQ=255)
                                             cAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt        >  1:2326050/1‑67 (MQ=255)
                                             cAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt        >  1:3089412/1‑67 (MQ=255)
                                               aTTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATg  <  1:1307328/71‑1 (MQ=255)
                                               aTTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATg  <  1:1512387/71‑1 (MQ=255)
                                               aTTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATg  <  1:213633/71‑1 (MQ=255)
                                               aTTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATg  <  1:8975/71‑1 (MQ=255)
                                               aTTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATg  <  1:900250/71‑1 (MQ=255)
                                                           tCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                        <  1:2212510/37‑1 (MQ=255)
                                                                  |                                                   
CCGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATG  >  minE/2516590‑2516707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑