| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,605,272 | A→G | L50L (CTT→CTC) | uspA ← | universal stress global response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,605,272 | 0 | A | G | 99.3% | 561.2 / ‑5.0 | 146 | L50L (CTT→CTC) | uspA | universal stress global response regulator |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (77/68); minor base T (0/1); total (77/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 146 total aligned reads displayed.
AGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAGGG > minE/2605213‑2605337 | aGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGta < 1:2739716/71‑1 (MQ=255)aGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGta < 1:941146/71‑1 (MQ=255)aGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGta < 1:578813/71‑1 (MQ=255)aGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGta < 1:2912115/71‑1 (MQ=255)aGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGta < 1:253134/71‑1 (MQ=255)aGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGta < 1:2353398/71‑1 (MQ=255)aGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGta < 1:1903498/71‑1 (MQ=255)aGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGta < 1:1058313/71‑1 (MQ=255) cATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAgg > 1:755186/1‑71 (MQ=255) cATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAgg > 1:2456797/1‑71 (MQ=255) cATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAgg > 1:1315726/1‑71 (MQ=255) cATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAgg > 1:543984/1‑71 (MQ=255) cATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAgg > 1:2676540/1‑71 (MQ=255) cATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAgg > 1:2789532/1‑71 (MQ=255) cATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAg > 1:2255871/1‑70 (MQ=255) tgatgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATTAGCCCGGTGTATAg < 1:3727199/68‑1 (MQ=255) tgatgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAg < 1:3712142/68‑1 (MQ=255) tgatgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAg < 1:3637697/68‑1 (MQ=255) tgatgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAg < 1:3090853/68‑1 (MQ=255) tgatgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAg < 1:4159927/68‑1 (MQ=255) tgatgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAg < 1:1021927/68‑1 (MQ=255) tgatgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAg < 1:4294535/68‑1 (MQ=255) tgatgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAg < 1:691443/68‑1 (MQ=255) tgatgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAg < 1:2413174/68‑1 (MQ=255) tgatgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAg < 1:2364403/68‑1 (MQ=255) tgatgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAg < 1:2809906/68‑1 (MQ=255) tgatgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAg < 1:1711013/68‑1 (MQ=255) tgatgTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGCGTATAg < 1:564740/68‑1 (MQ=255) tctcTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGagtag > 1:3171388/1‑71 (MQ=255) tctcTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGagtag > 1:3381676/1‑71 (MQ=255) tctcTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGagtag > 1:3676676/1‑71 (MQ=255) tctcTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGagtag > 1:1498482/1‑71 (MQ=255) tctcTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGagtag > 1:2956365/1‑71 (MQ=255) tctcTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGagtag > 1:2187920/1‑71 (MQ=255) tctcTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGagtag > 1:74336/1‑71 (MQ=255) tctcTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGagtag > 1:1280671/1‑71 (MQ=255) cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGc > 1:2622872/1‑44 (MQ=255) cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGgtgt > 1:2164489/1‑51 (MQ=255) cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGTTGTATAgg > 1:1896683/1‑56 (MQ=255) cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTAc < 1:4379386/70‑1 (MQ=255) cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTAc < 1:288438/70‑1 (MQ=255) cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTAc < 1:812464/70‑1 (MQ=255) cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTAc > 1:3792250/1‑70 (MQ=255) cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACa > 1:2856438/1‑71 (MQ=255) cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACa > 1:1241564/1‑71 (MQ=255) cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACa > 1:3797006/1‑71 (MQ=255) cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACa > 1:2217748/1‑71 (MQ=255) tatGCATATCACCCAGATTCACATCAATGAGCCCGGTGta < 1:4106166/38‑1 (MQ=38) tCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGAt > 1:328505/1‑70 (MQ=255) tCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGAt > 1:1707049/1‑70 (MQ=255) tCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGAt > 1:1550258/1‑70 (MQ=255) tCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGAt > 1:1263790/1‑70 (MQ=255) tGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAg < 1:1824814/41‑1 (MQ=255) atCACCCAGATTCGCATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGaaa > 1:4034563/1‑71 (MQ=255) atCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTCCGTGGATCAGAGaaa > 1:3390960/1‑71 (MQ=255) atCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCaga > 1:1193372/1‑67 (MQ=255) atCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGaaa > 1:3141374/1‑71 (MQ=255) atCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGaaa > 1:2334963/1‑71 (MQ=255) atCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGaaa > 1:1630126/1‑71 (MQ=255) atCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGaaa > 1:3250279/1‑71 (MQ=255) atCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGaaa > 1:4128579/1‑71 (MQ=255) atCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGaaa > 1:1323661/1‑71 (MQ=255) atCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGaaa > 1:3054941/1‑71 (MQ=255) atCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGaaa > 1:1094133/1‑71 (MQ=255) atCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGaaa > 1:885517/1‑71 (MQ=255) tCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAAc > 1:3939258/1‑71 (MQ=255) tCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAAc < 1:2051726/71‑1 (MQ=255) tCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAAc < 1:1986815/71‑1 (MQ=255) tCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAAc < 1:1540750/71‑1 (MQ=255) tCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAAc < 1:1233095/71‑1 (MQ=255) aCCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACt < 1:1283183/70‑1 (MQ=255) aCCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACt < 1:1337436/70‑1 (MQ=255) aCCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACt < 1:3527256/70‑1 (MQ=255) aCCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACt < 1:2774295/70‑1 (MQ=255) aCCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACt < 1:1911878/70‑1 (MQ=255) aCCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACt < 1:1593164/70‑1 (MQ=255) aCCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACt < 1:1426197/70‑1 (MQ=255) aGATTCACATCAATGAGCCCGGTGTATATGTCAGAGTAGTTTACATCTACGTGGAGCAGAGAAACTTTCGc < 1:4029030/71‑1 (MQ=255) aGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGc < 1:67694/71‑1 (MQ=255) aGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGc < 1:3558464/71‑1 (MQ=255) aGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGc < 1:936707/71‑1 (MQ=255) aGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGc < 1:11721/71‑1 (MQ=255) aGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGc < 1:3222969/71‑1 (MQ=255) aGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGc < 1:1325319/71‑1 (MQ=255) ttCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCAtt > 1:1950393/1‑71 (MQ=255) ttCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCAtt > 1:208521/1‑71 (MQ=255) ttCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCAtt > 1:828065/1‑71 (MQ=255) ttCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCAtt > 1:133031/1‑71 (MQ=255) ttCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCAtt > 1:222070/1‑71 (MQ=255) ttCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCAtt > 1:1413329/1‑71 (MQ=255) ttCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCAtt > 1:1226775/1‑71 (MQ=255) ttCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCAt > 1:1597258/1‑70 (MQ=255) acaTCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGt < 1:4364258/70‑1 (MQ=255) acaTCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGt < 1:2229364/70‑1 (MQ=255) tCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTAc > 1:4079142/1‑41 (MQ=255) tCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAggg > 1:249057/1‑71 (MQ=255) tCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAggg > 1:4260980/1‑71 (MQ=255) tCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAggg > 1:852872/1‑71 (MQ=255) tCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAggg > 1:2813100/1‑71 (MQ=255) tCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAgg > 1:1587958/1‑70 (MQ=255) | AGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAGGG > minE/2605213‑2605337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |