Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,632,970	A→G	K421R (AAA→AGA)	livM →	leucine/isoleucine/valine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,632,970	0	A	G	100.0%	178.2 / NA	47	K421R (AAA→AGA)	livM	leucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/21);  total (26/21)

GATCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGC  >  minE/2632902‑2633012                                                                     |                                           gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          >  1:3482847/1‑71 (MQ=255) gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          >  1:4318131/1‑71 (MQ=255) gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          >  1:2224937/1‑71 (MQ=255) gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          >  1:545425/1‑71 (MQ=255) gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          >  1:55591/1‑71 (MQ=255) gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          >  1:2127103/1‑71 (MQ=255) gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          >  1:4127511/1‑71 (MQ=255) gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          >  1:662362/1‑71 (MQ=255) gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          >  1:4042623/1‑71 (MQ=255) gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          >  1:396037/1‑71 (MQ=255) gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          >  1:3811800/1‑71 (MQ=255) gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          >  1:2917646/1‑71 (MQ=255) gatCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          >  1:372669/1‑71 (MQ=255)  atCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          <  1:4026022/70‑1 (MQ=255)  atCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          <  1:3784550/70‑1 (MQ=255)  atCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          <  1:3681771/70‑1 (MQ=255)  atCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          <  1:3628554/70‑1 (MQ=255)  atCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          <  1:4368234/70‑1 (MQ=255)  atCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          <  1:944822/70‑1 (MQ=255)  atCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          <  1:1100650/70‑1 (MQ=255)  atCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          <  1:1755720/70‑1 (MQ=255)  atCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          <  1:1387235/70‑1 (MQ=255)  atCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          <  1:852806/70‑1 (MQ=255)   tCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          <  1:3949453/69‑1 (MQ=255)       gCGTCCGCAGGGCTTGCTGCCCTTGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAgg                                         <  1:1569932/66‑1 (MQ=255)       gCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAgg                                         <  1:2122682/66‑1 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:3924297/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:92730/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:913465/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:3789215/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:3340146/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:3955482/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:730739/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:720391/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:4309752/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:481739/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:2701507/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:2348938/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:2464259/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:2473496/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:2507262/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:2061691/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:2179436/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:2883165/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:3134332/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:1370410/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:2343869/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAgg                                 >  1:1037223/1‑71 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAg                                  >  1:1556481/1‑70 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAg                                  >  1:31486/1‑70 (MQ=255)          tCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAg                                  >  1:3609464/1‑70 (MQ=255)            cGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCa                               >  1:640251/1‑71 (MQ=255)                  gCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGagag                                          <  1:4108028/54‑1 (MQ=255)                              tGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATtattat             <  1:2653230/71‑1 (MQ=255)                                   cgcCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGtt        <  1:3253671/71‑1 (MQ=255)                                   cgcCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGtt        <  1:1726537/71‑1 (MQ=255)                                   cgcCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGtt        <  1:1217786/71‑1 (MQ=255)                                   cgcCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGtt        <  1:2244146/71‑1 (MQ=255)                                   cgcCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGtt        <  1:4115232/71‑1 (MQ=255)                                   cgcCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGtt        <  1:3870535/71‑1 (MQ=255)                                   cgcCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGtt        <  1:366643/71‑1 (MQ=255)                                    gcCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGtt        <  1:2341030/70‑1 (MQ=255)                                         cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc  <  1:4313231/71‑1 (MQ=255)                                         cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc  <  1:2303007/71‑1 (MQ=255)                                         cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc  <  1:2180034/71‑1 (MQ=255)                                         cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc  <  1:3957613/71‑1 (MQ=255)                                         cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc  <  1:1603949/71‑1 (MQ=255)                                         cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc  <  1:3153854/71‑1 (MQ=255)                                         cAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc  <  1:1098690/71‑1 (MQ=255)                                          aaCTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc  <  1:224749/70‑1 (MQ=255)                                          aaCTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc  <  1:2754146/70‑1 (MQ=255)                                            cTGATGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGc  <  1:2584579/68‑1 (MQ=255)                                                                     |                                           GATCTGGCGTCCGCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCATGAGTCAGCCATTATTATCTGTTAACGGC  >  minE/2632902‑2633012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑