Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,681,918	A→G	R598R (CGT→CGC)	yhgF ←	predicted transcriptional accessory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,681,918	0	A	G	100.0%	331.6 / NA	89	R598R (CGT→CGC)	yhgF	predicted transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (56/33);  total (56/33)

ATGATGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATG  >  minE/2681856‑2681978                                                               |                                                             atgatgTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       <  1:2580886/70‑1 (MQ=255) atgatgTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       <  1:1273354/70‑1 (MQ=255) atgatgTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       <  1:1761933/70‑1 (MQ=255)    atgTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCgct                                                   <  1:924746/71‑1 (MQ=255)                    gCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGa                                   <  1:1011923/71‑1 (MQ=255)                    gCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGa                                   <  1:1829140/71‑1 (MQ=255)                    gCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGa                                   <  1:2836255/71‑1 (MQ=255)                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt                                       >  1:3184710/1‑66 (MQ=255)                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt                                       >  1:3274548/1‑66 (MQ=255)                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt                                       >  1:3038305/1‑66 (MQ=255)                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt                                       >  1:2888776/1‑66 (MQ=255)                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt                                       >  1:3833324/1‑66 (MQ=255)                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt                                       >  1:3919452/1‑66 (MQ=255)                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt                                       >  1:1609272/1‑66 (MQ=255)                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt                                       >  1:3924636/1‑66 (MQ=255)                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt                                       >  1:287132/1‑66 (MQ=255)                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt                                       >  1:1273276/1‑66 (MQ=255)                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt                                       >  1:1182980/1‑66 (MQ=255)                     cacaCCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAt                                       >  1:2557730/1‑66 (MQ=255)                                tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       >  1:2398878/1‑39 (MQ=255)                                tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       >  1:2754220/1‑39 (MQ=255)                                tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       >  1:3015774/1‑39 (MQ=255)                                tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       >  1:2287437/1‑39 (MQ=255)                                tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       >  1:2618454/1‑39 (MQ=255)                                tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       >  1:1993485/1‑39 (MQ=255)                                tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       >  1:3533948/1‑39 (MQ=255)                                tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       >  1:4124192/1‑39 (MQ=255)                                tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       >  1:4362716/1‑39 (MQ=255)                                tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       >  1:738088/1‑39 (MQ=255)                                tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt                                                       >  1:1099667/1‑39 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:3290374/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:302725/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:3506359/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:2874570/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:3608621/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:4062645/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:102492/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:2548810/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:2017571/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:1720/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:1674137/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:1402417/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:1399255/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:2292641/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:1187939/58‑1 (MQ=255)                                       aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc                             <  1:2442283/58‑1 (MQ=255)                                        gTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTTCTGTTACCCATCAGATCTTTc                             <  1:491150/57‑1 (MQ=255)                                            cggcAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgct           <  1:3374204/68‑1 (MQ=255)                                            aGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgct           <  1:712038/71‑1 (MQ=255)                                            aGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgct           <  1:3604063/71‑1 (MQ=255)                                              tCAGCCGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgctgc         >  1:2446756/1‑71 (MQ=255)                                              tCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCctgc                    >  1:2208591/1‑60 (MQ=255)                                              tCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgctgc         >  1:450861/1‑71 (MQ=255)                                              tCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgctgc         >  1:465766/1‑71 (MQ=255)                                              tCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgctgc         >  1:4308075/1‑71 (MQ=255)                                              tCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgctgc         >  1:3708660/1‑71 (MQ=255)                                              tCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgctgc         >  1:952597/1‑71 (MQ=255)                                              tCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgctgc         >  1:3566188/1‑71 (MQ=255)                                              tCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgctgc         >  1:1873906/1‑71 (MQ=255)                                              tCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgctgc         >  1:1993015/1‑71 (MQ=255)                                              tCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgctg          >  1:1166745/1‑70 (MQ=255)                                              acAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCAACAGa                                   >  1:1790452/2‑45 (MQ=38)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAg                                    >  1:3132435/1‑39 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:4199344/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:2745036/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:109272/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:882200/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:845077/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:2790517/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:697677/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:2352768/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:2345883/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:4350581/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:4313662/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:2229836/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:4069904/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:2220176/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:3039293/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:3182456/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:3771587/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:2163615/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa    >  1:3664323/1‑71 (MQ=255)                                                   cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGa     >  1:2966667/1‑70 (MQ=255)                                                     cTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATg  <  1:4107692/71‑1 (MQ=255)                                                     cTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATg  <  1:4057062/71‑1 (MQ=255)                                                     cTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATg  <  1:2815744/71‑1 (MQ=255)                                                     cTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATg  <  1:3306570/71‑1 (MQ=255)                                                      tttCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATg  <  1:1361461/70‑1 (MQ=255)                                                      tttCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATg  <  1:3435147/70‑1 (MQ=255)                                                               |                                                             ATGATGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATG  >  minE/2681856‑2681978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑