Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,726,174	G→A	R303R (CGC→CGT)	yheS ←	fused predicted transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,726,174	0	G	A	100.0%	154.0 / NA	51	R303R (CGC→CGT)	yheS	fused predicted transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/15);  total (36/15)

CGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGC  >  minE/2726113‑2726231                                                              |                                                          cGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCt                                                   >  1:1676602/1‑70 (MQ=255)      tAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTTAAGCg                                             >  1:2120233/1‑71 (MQ=255)      tAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCg                                             >  1:1971187/1‑71 (MQ=255)      tAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCg                                             >  1:2614177/1‑71 (MQ=255)      tAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCg                                             >  1:2617654/1‑71 (MQ=255)      tAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCg                                             >  1:3336658/1‑71 (MQ=255)      tAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCg                                             >  1:2173079/1‑71 (MQ=255)      tAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCg                                             >  1:2035003/1‑71 (MQ=255)      tAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCg                                             >  1:1740407/1‑71 (MQ=255)      tAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCg                                             >  1:1670564/1‑71 (MQ=255)                          cATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGc                                                    >  1:3452822/1‑44 (MQ=255)                          cATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTgcg                         >  1:1642899/1‑71 (MQ=255)                          cATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTgcg                         >  1:609675/1‑71 (MQ=255)                          cATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTgcg                         >  1:1066052/1‑71 (MQ=255)                                cAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCggg                     <  1:2481615/69‑1 (MQ=255)                                 aGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGc                  <  1:3891543/71‑1 (MQ=255)                                 aGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGc                  <  1:2046657/71‑1 (MQ=255)                                 aGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGc                  <  1:164841/71‑1 (MQ=255)                                 aGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGc                  <  1:15333/71‑1 (MQ=255)                                        gATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGc           <  1:3454028/71‑1 (MQ=255)                                        gATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGc           <  1:2473635/71‑1 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCATGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:4140657/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCgg                                            >  1:3896282/1‑36 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCt          >  1:706529/1‑70 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:2462982/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:1518191/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:531794/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         <  1:4301284/71‑1 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:4193309/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         <  1:1666957/71‑1 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:4110640/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:1875964/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:1895701/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:3632206/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:349795/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:211972/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:223166/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:3388584/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:3246536/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:2962830/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:2909936/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:2662590/1‑71 (MQ=255)                                          tttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc         >  1:2485162/1‑71 (MQ=255)                                             ggctggctTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCa       <  1:4147176/70‑1 (MQ=255)                                             ggCAGGCTTTCCGGCTCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCa       <  1:3478849/70‑1 (MQ=255)                                             ggCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCa       <  1:2458988/70‑1 (MQ=255)                                             ggCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCa       <  1:4147173/70‑1 (MQ=255)                                             ggCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCa       <  1:1111505/70‑1 (MQ=255)                                                 ggCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCa       <  1:524581/66‑1 (MQ=255)                                                 ggCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGc  >  1:3461208/1‑71 (MQ=255)                                                 ggCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGc  >  1:53579/1‑71 (MQ=255)                                                              |                                                          CGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGC  >  minE/2726113‑2726231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑