Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	575,416	T→C	N135S (AAT→AGT)	ybjX ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	575,416	0	T	C	100.0%	111.5 / NA	30	N135S (AAT→AGT)	ybjX	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

GTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCG  >  minE/575362‑575454                                                       |                                       gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:95658/71‑1 (MQ=255) gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:2353013/71‑1 (MQ=255) gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:237867/71‑1 (MQ=255) gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:4006485/71‑1 (MQ=255) gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:3582488/71‑1 (MQ=255) gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:2615217/71‑1 (MQ=255) gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:3261918/71‑1 (MQ=255) gTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGaa                        <  1:3112523/71‑1 (MQ=255)    aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:3219132/1‑71 (MQ=255)    aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:869968/1‑71 (MQ=255)    aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:574174/1‑71 (MQ=255)    aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:4284395/1‑71 (MQ=255)    aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:3588207/1‑71 (MQ=255)    aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:2692881/1‑71 (MQ=255)    aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:2483613/1‑71 (MQ=255)    aaCTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAt                       >  1:1789582/1‑69 (MQ=255)                tttttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGaca          >  1:2883176/1‑70 (MQ=255)                  tttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:1650175/1‑57 (MQ=255)                  tttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:2458425/1‑57 (MQ=255)                  tttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:2181817/1‑57 (MQ=255)                  tttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:791414/1‑57 (MQ=255)                  tttGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAAttc                     >  1:203170/1‑57 (MQ=255)                    tGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGacataca      <  1:3217362/70‑1 (MQ=255)                     gCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGacataca      <  1:4147938/69‑1 (MQ=255)                       cTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcg  <  1:4017219/71‑1 (MQ=255)                       cTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcg  <  1:857666/71‑1 (MQ=255)                       cTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcg  <  1:500765/71‑1 (MQ=255)                       cTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcg  <  1:3961477/71‑1 (MQ=255)                       cTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCGCCGACATACATTcg  <  1:2587719/71‑1 (MQ=255)                       cTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTACTCAAATATAAGCTGAATTCTTCCACCGACATACATTcg  <  1:1654788/71‑1 (MQ=255)                                                       |                                       GTGAACTGCTCGCCGTTTTTGCCTTCCAGCTTCGCCAGTTGCAGCCCGGGGGTATTCAAATATAAGCTGAATTCTTCCGCCGACATACATTCG  >  minE/575362‑575454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑