Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,802,519	T→A	D196V (GAT→GTT)	qor ←	quinone oxidoreductase, NADPH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,802,519	0	T	A	100.0%	192.9 / NA	62	D196V (GAT→GTT)	qor	quinone oxidoreductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (54/8);  total (54/8)

TGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGG  >  minE/2802451‑2802585                                                                     |                                                                   tGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAAcc                                                                  >  1:4170310/1‑71 (MQ=255) tGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAAcc                                                                  >  1:3803575/1‑71 (MQ=255) tGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAAcc                                                                  >  1:783793/1‑71 (MQ=255)         gAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                           >  1:2718193/1‑70 (MQ=255)         gAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                           >  1:809341/1‑70 (MQ=255)         gAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                           >  1:2773708/1‑70 (MQ=255)         gAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                           >  1:2986072/1‑70 (MQ=255)         gAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                           >  1:3052786/1‑70 (MQ=255)         gAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                           >  1:2124473/1‑70 (MQ=255)         gAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                           >  1:2055405/1‑70 (MQ=255)         gAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                           >  1:887565/1‑70 (MQ=255)         gAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                           >  1:3495448/1‑70 (MQ=255)         gAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                           >  1:143367/1‑70 (MQ=255)         gAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                           >  1:3763614/1‑70 (MQ=255)         gAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCGTCAc                                                           >  1:3178109/1‑70 (MQ=255)                              ttCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCa                                                            >  1:3761629/1‑48 (MQ=255)                              ttCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCAcgc                                     >  1:3347762/1‑71 (MQ=255)                              ttCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCAcgc                                     >  1:653250/1‑71 (MQ=255)                              ttCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCAcgc                                     >  1:922342/1‑71 (MQ=255)                              ttCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCAcgc                                     >  1:1633838/1‑71 (MQ=255)                              ttCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCAcg                                      >  1:3654512/1‑70 (MQ=255)                              ttCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCAc                                       >  1:107606/1‑69 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaat                                                 <  1:531537/44‑1 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaat                                                 <  1:2414326/44‑1 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaat                                                 <  1:2370265/44‑1 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaat                                                 <  1:1196282/44‑1 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaat                                                 <  1:2203270/44‑1 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaat                                                 <  1:2079504/44‑1 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaat                                                 <  1:3373481/44‑1 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaat                                                 <  1:3509255/44‑1 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgc                      >  1:489503/1‑71 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgc                      >  1:382990/1‑71 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgc                      >  1:892612/1‑71 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgc                      >  1:1449319/1‑71 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgc                      >  1:3139261/1‑71 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgc                      >  1:2249852/1‑71 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgc                      >  1:2957698/1‑71 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgc                      >  1:2440951/1‑71 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaataa                                               >  1:1791876/1‑44 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaataa                                               >  1:3458384/1‑44 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaataa                                               >  1:3095286/1‑44 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaataa                                               >  1:3799924/1‑44 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaataa                                               >  1:862804/1‑44 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaataa                                               >  1:3914047/1‑44 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaataa                                               >  1:649561/1‑44 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaataa                                               >  1:599798/1‑44 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaataa                                               >  1:4067183/1‑44 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaataa                                               >  1:2719209/1‑44 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaata                                                >  1:3400028/1‑43 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAAcc                                             >  1:560834/1‑46 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgcgc                    >  1:3647457/1‑71 (MQ=255)                                               ctctTTGAACCGCTCGACCAGAACCTCTTCACGATAGTtaataa                                               >  1:555732/1‑44 (MQ=38)                                                    ttAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTgc               >  1:483033/1‑71 (MQ=255)                                                    ttAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTgc               >  1:4032900/1‑71 (MQ=255)                                                           cTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGTGCGCTCTGCGCttttt        >  1:4264287/1‑71 (MQ=255)                                                           cTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCttttt        >  1:1152693/1‑71 (MQ=255)                                                                 cAGAACCTCTTCACGATAGTTAATAACCTGCCAcg                                      >  1:1407698/1‑35 (MQ=255)                                                                 cAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCgg  >  1:3999480/1‑71 (MQ=255)                                                                 cAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCgg  >  1:3043076/1‑71 (MQ=255)                                                                 cAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCgg  >  1:2724239/1‑71 (MQ=255)                                                                 cAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCgg  >  1:2249974/1‑71 (MQ=255)                                                                 cAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCgg  >  1:1916933/1‑71 (MQ=255)                                                                     |                                                                   TGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGCGCGG  >  minE/2802451‑2802585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑