Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	668,428	C→A	T589K (ACG→AAG)	uup →	fused predicted transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	668,428	0	C	A	100.0%	135.4 / NA	43	T589K (ACG→AAG)	uup	fused predicted transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/12);  total (31/12)

GCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTG  >  minE/668378‑668487                                                   |                                                            gcTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGcttcct                                         <  1:3589492/71‑1 (MQ=255) gcTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGcttcct                                         <  1:4351163/71‑1 (MQ=255)      cGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCttcttc                                    <  1:4112299/71‑1 (MQ=255)      cGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCttcttc                                    <  1:4000990/71‑1 (MQ=255)      cGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCttcttc                                    <  1:1855628/71‑1 (MQ=255)      cGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCttcttc                                    <  1:2038298/71‑1 (MQ=255)      cGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCttcttc                                    <  1:3630971/71‑1 (MQ=255)      cGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCttcttc                                    <  1:3693028/71‑1 (MQ=255)                   aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCGGCCGCAt                        >  1:3973518/1‑70 (MQ=255)                   aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:3917225/1‑70 (MQ=255)                   aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:3430297/1‑70 (MQ=255)                   aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:3418618/1‑70 (MQ=255)                   aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:312790/1‑70 (MQ=255)                   aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:2976583/1‑70 (MQ=255)                   aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:2406268/1‑70 (MQ=255)                   aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:2328965/1‑70 (MQ=255)                   aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:1397024/1‑70 (MQ=255)                   aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:1299029/1‑70 (MQ=255)                   aGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:1190981/1‑70 (MQ=255)                       cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCAt                        >  1:2782553/1‑66 (MQ=255)                       cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:3835578/1‑69 (MQ=255)                       cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:3638154/1‑69 (MQ=255)                       cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:834656/1‑69 (MQ=255)                       cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:3999906/1‑69 (MQ=255)                       cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:4322515/1‑69 (MQ=255)                       cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:2673706/1‑69 (MQ=255)                       cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:2858722/1‑69 (MQ=255)                       cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                     >  1:1285282/1‑69 (MQ=255)                       cTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTAAGTCAGCCGCATGAg                     >  1:379599/1‑69 (MQ=255)                          gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGa                 <  1:3830334/70‑1 (MQ=255)                          gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGa                 <  1:567872/70‑1 (MQ=255)                          gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGa                 <  1:2199908/70‑1 (MQ=255)                          gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGa                 <  1:3987675/70‑1 (MQ=255)                               gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:1214085/1‑70 (MQ=255)                               gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:682886/1‑70 (MQ=255)                               gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:490764/1‑70 (MQ=255)                               gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:1380406/1‑70 (MQ=255)                               gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:1846082/1‑70 (MQ=255)                               gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:2283530/1‑70 (MQ=255)                               gAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCaa            >  1:2437801/1‑70 (MQ=255)                                        aGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgcttg  >  1:1786893/1‑71 (MQ=255)                                        aGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgcttg  >  1:2453220/1‑71 (MQ=255)                                        aGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGtgctt   >  1:2113190/1‑70 (MQ=255)                                                   |                                                            GCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTG  >  minE/668378‑668487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑