Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,548,141	(C)9→8	intergenic (+243/‑123)	fadL → / → yfdF	long‑chain fatty acid outer membrane transporter/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,548,133	0	C	.	100.0%	61.1 / NA	15	intergenic (+235/‑131)	fadL/yfdF	long‑chain fatty acid outer membrane transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (0/15);  total (0/15)

TTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACA  >  minE/1548075‑1548162                                                           |                              ttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccctgg                     >  1:2480372/1‑66 (MQ=255)                 gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:1050834/71‑1 (MQ=255)                 gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:1090061/71‑1 (MQ=255)                 gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:1325508/71‑1 (MQ=255)                 gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:1330437/71‑1 (MQ=255)                 gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:1388149/71‑1 (MQ=255)                 gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:247599/71‑1 (MQ=255)                 gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:2483858/71‑1 (MQ=255)                 gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:2536602/71‑1 (MQ=255)                 gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:282475/71‑1 (MQ=255)                 gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:3287824/71‑1 (MQ=255)                 gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:413832/71‑1 (MQ=255)                 gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:610770/71‑1 (MQ=255)                 gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:747115/71‑1 (MQ=255)                  tCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:1260347/70‑1 (MQ=255)                  tCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca  <  1:893159/70‑1 (MQ=255)                                                           |                              TTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACA  >  minE/1548075‑1548162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑