Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,695,690	Δ1 bp	coding (320/741 nt)	yfhQ ←	predicted methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,695,687	0	G	.	100.0%	84.2 / NA	20	coding (323/741 nt)	yfhQ	predicted methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (18/2);  total (18/2)

TCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACG  >  minE/1695631‑1695710                                                         |                        tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCt           <  1:570596/70‑1 (MQ=255) tCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCt           <  1:511417/70‑1 (MQ=255)         tctTCATTGGTTAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGAc   >  1:508645/1‑70 (MQ=39)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGa    >  1:3029897/1‑69 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGAc   >  1:834815/1‑70 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:483892/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:973208/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:934515/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:923703/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:818833/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:570896/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:1010696/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:3195261/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:3165360/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:2866596/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:2861030/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:2838721/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:2465501/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:24641/1‑71 (MQ=255)         tctTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACC‑GGGTATTTGCCGCTTCAGCGACg  >  1:2278341/1‑71 (MQ=255)                                                         |                        TCTGCAACTCTTCATTGGTCAAGCCGACGCGCTCGCGACCAAACACCAGCGCCACCGGGGTATTTGCCGCTTCAGCGACG  >  minE/1695631‑1695710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑