Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,279,749	Δ1 bp	coding (633/1101 nt)	trmA →	tRNA (uracil‑5‑)‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,279,746	0	A	.	100.0%	359.1 / NA	80	coding (630/1101 nt)	trmA	tRNA (uracil‑5‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (55/25);  total (55/25)

CAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTCAAAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTAA  >  minE/2279684‑2279808                                                               |                                                               cAGCCGAACGCGGCGATGAATATTCAGCTGCTGGAATGGGCGCTGGACGTAACCAAAGGCTCaaaggc                                                           >  1:1545239/1‑65 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGa                                                        >  1:2911995/1‑70 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:2869730/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:1701842/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:1786172/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:1786630/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:1848823/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:2011991/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:2604943/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:2685871/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:1046447/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:2903881/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:2985080/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:3241436/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:598013/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:722033/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:767365/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:963020/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:1660402/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:1053916/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:1291193/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:10588/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:1167606/1‑71 (MQ=255) cAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTCGACGTAACCAAAGGCTC‑AAAGGCGAt                                                       >  1:282964/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:2385842/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:1030449/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:2743082/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:2849827/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:3009881/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:3061058/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:32324/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:1153790/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:3282978/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:468865/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:48152/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:622030/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:76304/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:789438/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:854871/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:883221/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:940496/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:2258814/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:2008418/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:1978346/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:2139568/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:2154953/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:1566145/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:1829648/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:1812743/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:237602/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:1603306/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:179647/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:2354211/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:2298678/1‑71 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGgc        >  1:1320773/1‑69 (MQ=255)                                                  tAACCAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGAGGCAACGGTAACTTTTCATTAGCGCTGgcgc      >  1:640100/1‑71 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACTGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:318957/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:1910099/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:1661117/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:739268/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:1937038/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:649808/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:62944/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:916455/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:1135614/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:1526278/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:2036375/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:2062718/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:3180005/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:2218886/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:2244704/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:2966108/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:12140/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:2890157/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:227549/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:2779953/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:2712284/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:1385787/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:2680938/71‑1 (MQ=255)                                                      cAAAGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:1421150/71‑1 (MQ=255)                                                        aaGGCTC‑AAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTaa  <  1:2853209/69‑1 (MQ=255)                                                               |                                                               CAGCCGAACGCGGCGATGAATATTCAGATGCTGGAATGGGCGCTGGACGTAACCAAAGGCTCAAAAGGCGATTTACTGGAGCTGTACTGCGGCAACGGTAACTTTTCATTAGCGCTGGCGCGTAA  >  minE/2279684‑2279808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑