Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	326,795	Δ1 bp	coding (272/963 nt)	hemH →	ferrochelatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	326,793	0	T	.	100.0%	143.0 / NA	33	coding (270/963 nt)	hemH	ferrochelatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (33/0);  total (33/0)

CAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCAC  >  minE/326770‑326842                        |                                                  cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCAt     >  1:1503347/1‑69 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATc    >  1:2852772/1‑70 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:2270716/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:860915/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:2330113/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:2335284/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:2444919/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:289030/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:2968400/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:3028092/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:311522/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:3187978/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:3295465/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:532426/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:710987/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:776731/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:1375884/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:2234334/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:2226145/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:2147019/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:1932375/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:1906973/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:1883374/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:1877992/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:1818885/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:1796932/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:1765867/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:1763550/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:1692204/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:161651/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCa   >  1:159071/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTC‑CCATCAc  >  1:435795/1‑71 (MQ=255) cAACAGCAGGCGCTGGCACAACG‑TTACCGGAGATGCCCGTAGCGCTGGGAATGAACTACGGCTCGCCATCa   >  1:2223299/1‑71 (MQ=255)                        |                                                  CAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCCGTAGCGCTGGGAATGAGCTACGGCTCGCCATCAC  >  minE/326770‑326842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑