Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,269,468	(A)7→8	intergenic (‑18/‑282)	uvrC ← / → yedP	excinuclease UvrABC, endonuclease subunit/conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,269,461	1	.	A	100.0%	19.4 / NA	8	intergenic (‑11/‑289)	uvrC/yedP	excinuclease UvrABC, endonuclease subunit/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (8/0);  total (8/0)

GCCGTCGCCGCATTGGTGCGCGCGAGTTTCAGATAACGCGCCCTATCGCCGGAATGCACAAT‑AAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAC  >  minE/1269400‑1269516                                                               |                                                        gCCGTCGCCGCATTGGTGCGCGCGAGTTTCAGATAACGCGCCCTATCGCCGGAATGCACAAT‑aaaaaaaat                                                >  1:1703394/1‑69 (MQ=255) gCCGTCGCCGCATTGGTGCGCGCGAGTTTCAGATAACGCGCCCTATCGCCGGAATGCACAAT‑aaaaaaaat                                                >  1:2112501/1‑69 (MQ=255)                                                gCCGGAATGCACAATAAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAc  >  1:1221271/1‑71 (MQ=255)                                                gCCGGAATGCACAATAAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAc  >  1:1933150/1‑71 (MQ=255)                                                gCCGGAATGCACAATAAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAc  >  1:2230795/1‑71 (MQ=255)                                                gCCGGAATGCACAATAAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAc  >  1:271860/1‑71 (MQ=255)                                                gCCGGAATGCACAATAAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAc  >  1:2753184/1‑71 (MQ=255)                                                gCCGGAATGCACAATAAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAc  >  1:35250/1‑71 (MQ=255)                                                gCCGGAATGCACAATAAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAc  >  1:723959/1‑71 (MQ=255)                                                gCCGGAATGCACAATAAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAc  >  1:981031/1‑71 (MQ=255)                                                               |                                                        GCCGTCGCCGCATTGGTGCGCGCGAGTTTCAGATAACGCGCCCTATCGCCGGAATGCACAAT‑AAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAC  >  minE/1269400‑1269516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑