Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,508,224	+A	coding (357/495 nt)	yfbU ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,508,220	1	.	A	100.0%	101.2 / NA	30	D121Y (GAC→TAC)	yfbU	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/30);  total (0/30)

TTCCGGCGTC‑AAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGC  >  minE/1508211‑1508280           |                                                             ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGGGGACATAACCGAGGTAACGTGc  <  1:771045/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:3020312/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:988640/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:923165/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:820584/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:781869/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:749932/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:606051/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:454420/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:443669/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:416102/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:3172018/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:315327/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:3082433/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:3073549/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:1239842/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:2839332/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:2711983/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:2482317/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:214296/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:1902436/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:1704496/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:1701091/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:1581128/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:1471833/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:134436/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:1291861/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:1269076/71‑1 (MQ=255) ttCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:1247479/71‑1 (MQ=255)  tCCGGCGTCAAAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGc  <  1:2433862/70‑1 (MQ=255)           |                                                             TTCCGGCGTC‑AAAATGGGTATAGCGCCCTTCCACATTAACCATAAAGCGGACATAACCGAGGTAACGTGC  >  minE/1508211‑1508280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑