| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 717,230 | 0 | G | A | 71.1% | 66.3 / 33.2 | 45 | E82E (GAG→GAA) | yceH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (10/3); new base A (16/16); total (26/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCAC > minE/717163‑717295 | aTAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCaa > 1:731974/1‑71 (MQ=255)aTAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCaa > 1:151261/1‑71 (MQ=255)aTAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGCAATCGGGTCACCAAATATGAGCaa > 1:1290321/1‑71 (MQ=255) cTGGTCAAACGTCATTATCTACTCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:278361/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCTCCAAATATGAg > 1:1718413/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:3265765/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:602774/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:550782/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:429493/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:34546/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:1978571/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:327636/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:2116106/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:1024244/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:2187370/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:2211861/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:2329714/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:236302/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:2406945/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:2961619/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:2715863/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:1863756/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:1173035/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:1008027/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:925950/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:915087/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:1375042/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:806403/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:802643/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:1619399/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:780007/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:1667021/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:1783298/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAg > 1:635874/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTCGTAATCGGGTCACCAAATATGAg > 1:1921635/1‑63 (MQ=255) cTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTATGGTAATCGGGTCACCAAATATGAg > 1:3142892/1‑63 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTaa > 1:661247/4‑68 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:327632/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:3247634/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:929201/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:3111631/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:2831169/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:96016/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:1816543/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:2657592/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:1635595/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:1956637/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:2562564/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:2385938/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:2070471/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTTTTTGTAAtt > 1:1379576/4‑70 (MQ=255) cgccATTATCTGCGCACGGTGAGTGGCTTTGGTAATCGGGTCACCAAATATGAACAACGTATTTGTAAtt > 1:1316568/4‑70 (MQ=255) aTCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGa < 1:1331204/70‑1 (MQ=255) aTCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGa < 1:2269687/70‑1 (MQ=255) aTCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGa < 1:2154795/70‑1 (MQ=255) tCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGaa > 1:1359975/1‑70 (MQ=255) tCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAg > 1:2680930/1‑71 (MQ=255) tCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAg > 1:1819956/1‑71 (MQ=255) tCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAg > 1:1479605/1‑71 (MQ=255) tCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAg > 1:1970730/1‑71 (MQ=255) tCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAg > 1:2931232/1‑71 (MQ=255) tCGGGTCACCAAATATGAGCAACGTTTTTATAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAg > 1:649624/1‑71 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:879847/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:931625/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:1637731/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:1240668/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:2678255/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:722962/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:1954470/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:195567/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:3292399/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:3268871/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:2185020/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:3079802/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:3012152/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:2566699/71‑1 (MQ=255) tatGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:2916895/71‑1 (MQ=255) atGAACAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCCGCAGAAGTGGCGTTAATcac < 1:360193/70‑1 (MQ=255) | ATAATCTGGTCAAACGTCATTATCTACGCACAGTGAGCGGTTTTGGTAATCGGGTCACCAAATATGAGCAACGTTTTTGTAATTCAGAATTTGGCGATCTGAAACTGAGCGCAGCGGAAGTGGCGTTAATCAC > minE/717163‑717295 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |