| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 773,908 | 1 | . | G | 72.4% | 69.3 / 25.0 | 30 | coding (299/654 nt) | ymfC | 23S rRNA pseudouridine synthase |
| Reads supporting (aligned to +/- strand): ref base . (8/0); new base G (21/0); total (30/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCT‑GGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAA > minE/773863‑773954 | ccTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCT‑GGGTTAAACGCGCCTGCAACGCGcc > 1:2884585/1‑71 (MQ=255)ccTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCT‑GGGTTAAACGCGCCTGCAACGCGcc > 1:3260917/1‑71 (MQ=255)ccTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCT‑GGGTTAAACGCGCCTGCAACGCGcc > 1:358491/1‑71 (MQ=255)ccTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCT‑GGGTTAAACGCGCCTGCAACGCGcc > 1:1329839/1‑71 (MQ=255)ccTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCT‑GGGTTAAACGCGCCTGCAACGCGcc > 1:37863/1‑71 (MQ=255)ccTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCT‑GGGTTAAACGCGCCTGCAACGCGcc > 1:1720652/1‑71 (MQ=255)ccTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCT‑GGGTTAAACGCGCCTGCAACGCGcc > 1:1493108/1‑71 (MQ=255)ccTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCAGGCT‑GGGTTAAACGCGCCTGCAATGCGcc > 1:311509/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTg > 1:1364207/1‑57 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCa > 1:2845848/1‑64 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAg > 1:1256269/1‑68 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:479567/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:480429/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:673990/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:3238381/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:3205519/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:889046/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:978823/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:3070582/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:2935291/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:2640027/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:2434523/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:1831581/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:144295/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:1334114/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:1235624/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:1211581/1‑71 (MQ=255) tttttCCGGTGCGTTTACCCGGCTCGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCaa > 1:2305849/1‑71 (MQ=255) tttttCCGGTGCGTTTACACGGCTGGGGTTAAACGCGCCTg > 1:1064546/1‑41 (MQ=255) tttttCCCGTGCGTTTACCCGGCTGGGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCa > 1:2337046/1‑70 (MQ=255) | CCTTCCACCTGCACATAATAGATTTTTCCGGTGCGTTTACCCGGCT‑GGGTTAAACGCGCCTGCAACGCGCCGTTATTGGTCAGCACCAGCAA > minE/773863‑773954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |