| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 946,598 | 1 | . | C | 60.0% | 16.4 / 10.8 | 10 | coding (987/1551 nt) | ddpA | D‑Ala‑D‑Ala transporter subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/4); new base C (0/6); total (0/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGTCGCATCGTAG‑CCCCACATGCCTTCCGGAATCGGGCCGCGCATCTGTTTTCCGTTACCACTCAGAATG > minE/946585‑946655 | ccGTCGCATCGTAG‑CCCCACATGCCTTCCGGAATCGGGCCGCGCATCTGTTTTCCGTTACCACTCAGAAt < 1:1047279/70‑1 (MQ=255)ccGTCGCATCGTAG‑CCCCACATGCCTTCCGGAATCGGGCCGCGCATCTGTTTTCCGTTACCACTCAGAAt < 1:23760/70‑1 (MQ=255)ccGTCGCATCGTAG‑CCCCACATGCCTTCCGGAATCGGGCCGCGCATCTGTTTTCCGTTACCACTCAGAAt < 1:2514684/70‑1 (MQ=255)ccGTCGCATCGTAG‑CCCCACATGCCTTCCGGAATCGGGCCGCGCATCTGTTTTCCGTTACCACTCAGAAt < 1:2919481/70‑1 (MQ=255) cGTCGCATCGTAGCCCCCACATGCCTTCCGGAATCGGGCCGCGCATCTGTTTTCCGTTACCACTCAGAATg < 1:1241290/71‑1 (MQ=255) cGTCGCATCGTAGCCCCCACATGCCTTCCGGAATCGGGCCGCGCATCTGTTTTCCGTTACCACTCAGAATg < 1:1523387/71‑1 (MQ=255) cGTCGCATCGTAGCCCCCACATGCCTTCCGGAATCGGGCCGCGCATCTGTTTTCCGTTACCACTCAGAATg < 1:2736812/71‑1 (MQ=255) cGTCGCATCGTAGCCCCCACATGCCTTCCGGAATCGGGCCGCGCATCTGTTTTCCGTTACCACTCAGAATg < 1:3025893/71‑1 (MQ=255) cGTCGCATCGTAGCCCCCACATGCCTTCCGGAATCGGGCCGCGCATCTGTTTTCCGTTACCACTCAGAATg < 1:344166/71‑1 (MQ=255) cGTCGCATCGTAGCCCCCACATGCCTTCCGGAATCGGGCCGCGCATCTGTTTTCCGTTACCACTCAGAATg < 1:947170/71‑1 (MQ=255) | CCGTCGCATCGTAG‑CCCCACATGCCTTCCGGAATCGGGCCGCGCATCTGTTTTCCGTTACCACTCAGAATG > minE/946585‑946655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |