| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,901,601 | 0 | T | A | 60.0% | 83.6 / 157.8 | 125 | S480S (TCA→TCT) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base T (50/0); new base A (75/0); total (125/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 125 total aligned reads displayed.
GTTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATT > minE/1901557‑1901669 | gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGa > 1:1994370/1‑47 (MQ=255)gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGa > 1:1723038/1‑47 (MQ=255)gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGa > 1:255439/1‑47 (MQ=255)gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGa > 1:867162/1‑47 (MQ=255)gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGa > 1:14647/1‑47 (MQ=255)gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTAttt > 1:2791764/1‑70 (MQ=255)gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTAttt > 1:2657565/1‑70 (MQ=255)gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTc > 1:2538746/1‑71 (MQ=255)gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTc > 1:2081800/1‑71 (MQ=255)gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTc > 1:2975130/1‑71 (MQ=255)gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTc > 1:2242257/1‑71 (MQ=255)gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTc > 1:1330003/1‑71 (MQ=255)gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTc > 1:2436985/1‑71 (MQ=255)gtTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTc > 1:1075603/1‑71 (MQ=255)gtTTCACATTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTAttt > 1:1057727/1‑70 (MQ=255) tttCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTcg > 1:3076548/1‑71 (MQ=255) tttCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTcg > 1:3010723/1‑71 (MQ=255) tttCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTcg > 1:1041938/1‑71 (MQ=255) tttCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTc > 1:2167467/1‑70 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGGACGCGTCATCAGTc > 1:1814648/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGt > 1:2672116/1‑70 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2870708/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2797618/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2934310/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2935748/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2950145/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2632883/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2787920/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2651548/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:3235677/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:583472/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:101086/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:955678/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:93477/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:831120/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:805321/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:709967/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:690493/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:676561/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:641706/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:3108289/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:574723/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:556129/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:496942/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:429955/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:3250604/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:3237284/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:961602/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:3208311/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:3165589/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1590449/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2062919/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:204382/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1882213/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1873522/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1796973/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1707321/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1706350/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1656889/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1826499/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1542115/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1388430/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1329430/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1285164/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1219705/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1193037/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1016640/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2122144/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2086390/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2387899/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:216443/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2444015/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2191351/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2427119/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2410649/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCCTCATCAGt > 1:2195654/1‑70 (MQ=255) aCGCCTCATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:419518/1‑71 (MQ=255) gCTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:823335/1‑71 (MQ=255) gCTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:379493/1‑71 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGtc > 1:777197/1‑46 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:265262/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2592959/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:723564/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:1280698/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:1093859/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2689309/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2694305/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2701097/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:1691566/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2365655/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2330239/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:487710/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:1780431/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:384838/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:365796/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2526524/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:1842206/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:3197843/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2122866/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2052255/1‑70 (MQ=255) | GTTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATT > minE/1901557‑1901669 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |