| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,901,628 | 0 | G | A | 59.5% | 90.1 / 151.3 | 126 | R471R (CGC→CGT) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (48/3); new base A (75/0); total (123/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 130 total aligned reads displayed.
TTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCACGCTGTTCACCATTCCTGGTGCGGAAC > minE/1901558‑1901698 | tttCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTcg > 1:1041938/1‑71 (MQ=255)tttCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTcg > 1:3076548/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGt > 1:2672116/1‑70 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:3108289/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:3208311/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2998294/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2950145/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2934310/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2910087/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2870708/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2787920/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:101086/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2622392/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2444015/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2427119/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2387899/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2225044/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2191351/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:583472/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:961602/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:955678/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:93477/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:831120/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:805321/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:690493/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:676561/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:663287/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:3165589/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:574723/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:556129/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:496942/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:429955/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:3250604/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:3235677/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2651548/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1707321/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1016640/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1193037/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1219705/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:123869/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1329430/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1347456/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1388430/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1542115/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1641963/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1656889/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1710464/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1796973/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1818474/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1826499/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1873522/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1882213/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2122144/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2063658/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:2062919/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:1993919/1‑71 (MQ=255) aCGCCTGATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCCTCATCAGt > 1:2195654/1‑70 (MQ=255) aCGCCTAATTTTTGCCGGCAGATTTCACAGGAATAAACGGTATTTCACGAAACATGAACGCGTCATCAGTc > 1:417378/1‑71 (MQ=255) gCTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:379493/1‑71 (MQ=255) gCTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:823335/1‑71 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2741646/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2365655/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2330239/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:487710/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:1280698/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:384838/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:723564/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:732856/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:3239803/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2694305/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:3197843/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:1691566/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2122866/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2052255/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:265262/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:1780431/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2526524/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2592959/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:2701097/1‑70 (MQ=255) cTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTAtt > 1:1842206/1‑70 (MQ=255) tCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTc > 1:2767363/1‑65 (MQ=255) tCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCa > 1:1202341/1‑66 (MQ=255) tCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCa > 1:772046/1‑66 (MQ=255) tCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCa > 1:1126628/1‑66 (MQ=255) tCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCa > 1:275641/1‑66 (MQ=255) tCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCa > 1:1389784/1‑66 (MQ=255) tCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCa > 1:426741/1‑66 (MQ=255) tCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCa > 1:3274251/1‑66 (MQ=255) tCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCa > 1:154337/1‑66 (MQ=255) tCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCa > 1:2427618/1‑66 (MQ=255) tCACTGGAATAAACGGAATTTCGCGAAACATGAAcc > 1:2856480/1‑35 (MQ=39) ggTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCt < 1:2673397/46‑1 (MQ=255) ggTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCt < 1:2984548/46‑1 (MQ=255) tATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCt < 1:2336098/44‑1 (MQ=255) tATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCACGCTGTTCACCATTCCTGGTg > 1:1473524/1‑71 (MQ=255) tATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCACGCTGTTCACCATTCCTGGTg > 1:732489/1‑71 (MQ=255) tATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCACGCTGTTCACCATTCCTGGTg > 1:3175096/1‑71 (MQ=255) tATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCACGCTGTTCACCATTCCTGGTg > 1:1070273/1‑71 (MQ=255) tATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCACGATGTTCACCATTCCTGGTg > 1:2082053/1‑71 (MQ=255) gcgAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCACGCTGTTCACCATTCCTGGTGCGGAAc < 1:2157882/71‑1 (MQ=255) | TTTCACCTTTAAATACAAAACGTAACGCCTGATTTTTCCCGGCTGATTTCACTGGAATAAACGGTATTTCGCGAAACATGAACGCGTCATCAGTCTGGCTGAAAAGCTTATTCACGCTGTTCACCATTCCTGGTGCGGAAC > minE/1901558‑1901698 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |