| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,312,331 | 0 | C | A | 57.9% | ‑1.3 / 92.1 | 57 | A259E (GCG→GAG) | menA | 1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (22/2); new base A (0/33); total (22/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.91e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAATGGACCCGGTGGCGATGCGACCAA > minE/2312263‑2312399 | gCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:3268976/69‑1 (MQ=255)gCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:1828871/69‑1 (MQ=255)gCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:2616172/69‑1 (MQ=255)gCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:813255/69‑1 (MQ=255)gCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:1917227/69‑1 (MQ=255)gCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:1957313/69‑1 (MQ=255)gCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:2448802/69‑1 (MQ=255)gCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:381661/69‑1 (MQ=255)gCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:1258950/69‑1 (MQ=255)gCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:61033/69‑1 (MQ=255)gCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:3107690/69‑1 (MQ=255)gCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:1177951/69‑1 (MQ=255)gCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCGGCTggc < 1:420771/69‑1 (MQ=255)gCTGGTGTCTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:336033/69‑1 (MQ=255) cTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTTCTggc < 1:3053663/68‑1 (MQ=255) cgcTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGCGGCACCATTACTGGTg < 1:316260/71‑1 (MQ=255) cgcTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGCGGCACCATTACTGGTg < 1:1655574/71‑1 (MQ=255) tAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTggc < 1:1268825/48‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:558146/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:2472116/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:2221920/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:2383760/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:2476429/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:2537116/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:2638125/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:2730838/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:2802827/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:2956055/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:2959780/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:3292288/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:3273430/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:839545/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:1252910/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:1255475/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:1330045/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:1386700/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:1418694/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:849580/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:1480940/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:1525636/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:795055/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:1209581/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:2039064/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:63482/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:2370880/69‑1 (MQ=255) ttCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCACGTTa < 1:1493729/69‑1 (MQ=255) ttCGCTGCATAGACTGTGGGGCTGGCTGTTCATGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:1579206/69‑1 (MQ=255) ttCGCTGCAGAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:1460122/69‑1 (MQ=255) tCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:3155333/68‑1 (MQ=255) tCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:2508015/68‑1 (MQ=255) tgtgGGGCTGGCTGTTCCTGCTGGAGGCACCATTACTGGTGAAGCAAGCCCGTTa < 1:1008734/55‑1 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:894773/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:887729/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:534655/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:667629/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:2491592/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:605024/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:2367424/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:1131254/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:1154888/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:1541300/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:1561683/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:1648066/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:1804144/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:2187554/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:3227814/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:240341/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:2449648/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:257059/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:2955710/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:2986208/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:302809/1‑57 (MQ=255) gTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAAt > 1:3076835/1‑57 (MQ=255) ggcggcACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAATGGACCCGGTGGCGATGCGACCaa > 1:14504/1‑71 (MQ=255) | GCTGGTGTGTCTGGCGCTGTTTAATCTCTTTTCGCTGCATAGCCTGTGGGGCTGGCTGTTCCTGCTGGCGGCACCATTACTGGTGAAGCAAGCCCGTTATGTGATGCGGGAAATGGACCCGGTGGCGATGCGACCAA > minE/2312263‑2312399 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |