| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,444,818 | 0 | A | G | 65.8% | 41.8 / 37.0 | 41 | Y106Y (TAT→TAC) | ilvD | dihydroxyacid dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (6/8); new base G (27/0); total (33/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTTGTCGCAGTTAGAGATGCAGACCATGGCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGC > minE/2444763‑2444886 | ttttGTCGCAGTTAGAGATGCAGACCATGGCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCa < 1:2311824/71‑1 (MQ=255)ttttGTCGCAGTTAGAGATGCAGACCATGGCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCa < 1:2117765/71‑1 (MQ=255)ttttGTCGCAGTTAGAGATGCAGACCATGGCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCa < 1:210653/71‑1 (MQ=255)ttttGTCGCAGTTAGAGATGCAGACCATGGCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCa < 1:1996228/71‑1 (MQ=255)ttttGTCGCAGTTAGAGATGCAGACCATGGCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCa < 1:193333/71‑1 (MQ=255)ttttGTCGCAGTTAGAGATGCAGACCATGGCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCa < 1:2792154/71‑1 (MQ=255)ttttGTCGCAGTTAGAGATGCAGACCATGGCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCa < 1:2846677/71‑1 (MQ=255) gCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAAt > 1:3053455/1‑70 (MQ=255) gCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAAt > 1:1616880/1‑70 (MQ=255) gCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAAt > 1:308530/1‑70 (MQ=255) gCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAAt > 1:3138021/1‑70 (MQ=255) gCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAAt > 1:1400762/1‑70 (MQ=255) gCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGCAATCAGCGATCAGTTCGCGAGATGGCAGTGaa > 1:2456967/1‑69 (MQ=255) gtgggCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:1399664/4‑69 (MQ=255) gTCGGCGCAGTGGGCATTGCCCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:1598356/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGCCCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:424428/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACTATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:2139801/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGCCCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:1243555/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGaa > 1:2429584/1‑37 (MQ=18) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:846367/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:32170/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:767033/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:2581336/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:539084/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:2595675/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:514203/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:266595/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:2701768/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:3870/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:3275414/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:1105473/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:3136356/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:1254495/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:1497295/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:1520043/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:1362708/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:135704/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:1320142/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:1039183/1‑69 (MQ=255) gTCGGCGCAGTGGGCATTGACCATGTACTCGACCGAATCGGCGATCAGGTCGCGCGAGGGCAGCGAGTa > 1:2097968/1‑69 (MQ=255) cATATACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAAT‑AAGCATCCCCCCGTGGCCCATGGc < 1:2301081/71‑1 (MQ=255) atatACTCAACGTAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:669785/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:1210304/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:725871/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:960442/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:514912/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:83196/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:1355250/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:837086/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:334041/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:3231206/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:1759396/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:2449773/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:2502774/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:2029099/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:193956/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:2622301/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:2633214/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:1885985/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:3182292/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:1689788/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:2973367/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:1612834/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:1548962/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:3092231/71‑1 (MQ=255) atatACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:3121819/71‑1 (MQ=255) tataCTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:504809/70‑1 (MQ=255) tataCTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:1429837/70‑1 (MQ=255) ataCTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGc < 1:1846384/69‑1 (MQ=255) | TTTTGTCGCAGTTAGAGATGCAGACCATGGCGTCGGCGCAGTGGGCGTTGACCATATACTCAACGGAATCAGCGATCAGTTCGCGAGATGGCAGTGAATAAAGCATCCCCCCGTGGCCCATGGC > minE/2444763‑2444886 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |