Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,628,3650CT73.5% 200.5 / 80.3 114P279S (CCG→TCG) livJleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/30);  new base T (60/23);  total (60/54)
Fisher's exact test for biased strand distribution p-value = 2.92e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 114 total aligned reads displayed.

ACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTCGTTTG  >  minE/2628303‑2628432
                                                              |                                                                   
aCATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACGATCAGGTTCCGGCGaa                                                              <  1:1140264/70‑1 (MQ=255)
aCATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACGATCAGGTTCCGGCGaa                                                              <  1:801163/70‑1 (MQ=255)
aCATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACGATCAGGTTCCGGCGaa                                                              <  1:1359021/70‑1 (MQ=255)
   ttGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACGATCAGGTTTCGGCGAACaaa                                                          <  1:458037/71‑1 (MQ=255)
   ttGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACGATCAGGTTTCGGCGAACaaa                                                          <  1:532454/71‑1 (MQ=255)
   ttGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACGATCAGGTTTCGGCGAACaaa                                                          <  1:2325084/71‑1 (MQ=255)
   ttGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACGATCAGGTTTCGGCGAACaaa                                                          <  1:1262502/71‑1 (MQ=255)
           gAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACGATCAGGTTCCGGCGaa                                                              <  1:1987555/59‑1 (MQ=255)
              tCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACGATCAGGTTTCGGCGCACAAACCCAttgttg                                                >  1:2603629/1‑70 (MQ=255)
              tCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACGATCAGGTTTCGGCGAACAACCCCAttgtt                                                 >  1:288727/1‑69 (MQ=255)
                             ctgGTGACCAAGCCGAAGAACTACGATCAGGTTTCGGCGAACaaa                                                          <  1:1185172/45‑1 (MQ=255)
                                                   aCGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:756843/70‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:2969672/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:3026379/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:3055646/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:3079525/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:2913973/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:25566/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:2462754/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:219053/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:376438/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:2867224/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:2882540/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:1852631/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:1096814/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:724675/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:1321610/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:1688735/69‑1 (MQ=255)
                                                    cGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:1414159/69‑1 (MQ=255)
                                                     gATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:100978/68‑1 (MQ=255)
                                                     gATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGgcgc           <  1:645332/68‑1 (MQ=255)
                                                      atgaGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1399903/4‑71 (MQ=255)
                                                      aTCAGGTTTCTGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   >  1:968440/1‑59 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCaaa                                     >  1:1955345/1‑41 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAgg                        >  1:2730908/1‑54 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   >  1:3115846/1‑59 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCGAGTGGCGCATTc       >  1:535021/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAg                 >  1:350927/1‑61 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2975912/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:3039402/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:786205/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:817485/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:720861/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:3108421/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:3150160/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:507207/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:648634/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:3167429/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:641755/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:3179843/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:3199152/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:412414/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1026232/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:512312/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:462119/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:478303/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2863414/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1259086/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1360529/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1462008/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1463269/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1530329/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1547367/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1587500/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1594020/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1701407/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1793114/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:181291/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1831184/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:1920571/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2038464/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2054623/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2219078/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2105225/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2556968/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2898080/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2575089/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2858391/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2839601/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2599105/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2721573/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:2656530/1‑71 (MQ=255)
                                                      aTCAGGTTTCGGCGAACAAACCAATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTc       >  1:3145608/1‑71 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:991533/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:116711/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:946847/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:2989498/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:2673414/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:1627039/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:584674/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:1682590/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:2638387/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:1744807/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:1796446/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:3114708/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:441704/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:388788/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:2018529/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCa                   <  1:3236921/54‑1 (MQ=255)
                                                           gTTTCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTCGTTTg  >  1:2936163/1‑71 (MQ=255)
                                                              |                                                                   
ACATTGCGGGCGAATCAGCGGAAGGGCTGCTGGTGACCAAGCCGAAGAACTACGATCAGGTTCCGGCGAACAAACCCATTGTTGACGCGATCAAAGCGAAAAAACAGGACCCAAGTGGCGCATTCGTTTG  >  minE/2628303‑2628432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑