Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,753,142	0	A	C	60.6%	43.7 / 31.4	33	K83T (AAA→ACA)	zntR	DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (1/12);  new base C (20/0);  total (21/12)
Fisher's exact test for biased strand distribution p-value = 3.66e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTGGAGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAAAAGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCA  >  minE/2753075‑2753179                                                                    |                                      cTGGAGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAAAAgg                                    >  1:2469576/1‑71 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:1236503/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:884077/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:824917/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:792585/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:638286/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:327283/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:30346/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:2961285/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:2925470/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:2833218/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:2756333/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:2386170/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:1595894/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:1745631/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:2059320/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:2005777/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:1978952/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:1897321/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAtc                                >  1:1855878/1‑70 (MQ=255)     aGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAACAGGCAt                                 >  1:409618/1‑70 (MQ=255)                                   tCGATCCTGAACACCATACCTGTCAGGAGTCAAACGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCa  <  1:2594702/71‑1 (MQ=255)                                   tCGATCCTGAACACCATACCTGTCAGGAGTCAAAAGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCa  <  1:996500/71‑1 (MQ=255)                                   tCGATCCTGAACACCATACCTGTCAGGAGTCAAAAGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCa  <  1:2370784/71‑1 (MQ=255)                                   tCGATCCTGAACACCATACCTGTCAGGAGTCAAAAGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCa  <  1:1205773/71‑1 (MQ=255)                                   tCGATCCTGAACACCATACCTGTCAGGAGTCAAAAGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCa  <  1:1523462/71‑1 (MQ=255)                                   tCGATCCTGAACACCATACCTGTCAGGAGTCAAAAGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCa  <  1:648510/71‑1 (MQ=255)                                   tCGATCCTGAACACCATACCTGTCAGGAGTCAAAAGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCa  <  1:2105612/71‑1 (MQ=255)                                   tCGATCCTGAACACCATACCTGTCAGGAGTCAAAAGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCa  <  1:2751332/71‑1 (MQ=255)                                   tCGATCCTGAACACCATACCTGTCAGGAGTCAAAAGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCa  <  1:2647229/71‑1 (MQ=255)                                   tCGATCCTGAACACCATACCTGTCAGGAGTCAAAAGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCa  <  1:2123071/71‑1 (MQ=255)                                   tCGATCCTGAACACCATACCTGTCAGGAGTCAAAAGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCa  <  1:241034/71‑1 (MQ=255)                                   tCGATCCTGAACACCATACCTGTCAGGAGTCAAAAGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCa  <  1:2403811/71‑1 (MQ=255)                                                                    |                                      CTGGAGTCGATCCGCGAGTTGCTGTCGATCCGCATCGATCCTGAACACCATACCTGTCAGGAGTCAAAAGGCATTGTGCAGGAAAGATTGCAGGAAGTCGAAGCA  >  minE/2753075‑2753179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑