| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,902,091 | 0 | C | A | 74.2% | 40.8 / 23.7 | 31 | E652* (GAG→TAG) | nrdD | anaerobic ribonucleoside‑triphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/8); new base A (0/23); total (0/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAG > minE/2902031‑2902161 | gggAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTaaa < 1:1184777/70‑1 (MQ=255)gggAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTaaa < 1:1040359/70‑1 (MQ=255)gggAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTaaa < 1:866258/70‑1 (MQ=255) gTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTAAAGCCACACt < 1:923598/71‑1 (MQ=255) gTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTAAAGCCACACt < 1:2403705/71‑1 (MQ=255) gTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTAAAGCCACACt < 1:2178666/71‑1 (MQ=255) gTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTAAAGCCACACt < 1:2889021/71‑1 (MQ=255) gggCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTaaa < 1:2697960/44‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCTGTGTATTGGTGCCGTAATACGGTa < 1:1493867/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:330572/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:3016793/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:2990933/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:571469/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:2607427/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:2554600/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:696493/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:906986/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:2310164/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:245192/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:1976972/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:1827716/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:1670196/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:1589645/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:1489949/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:1422419/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:1385964/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:1287235/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:1265914/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:1195679/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTa < 1:1155546/70‑1 (MQ=255) cACTCGAACTAACCGGTAAAGCCACACTCGTAGCACTCATAAATCGGTGTATTGGTGCCGTAATACGGTa < 1:1505292/70‑1 (MQ=255) cACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAg > 1:2521465/1‑71 (MQ=255) | GGGAGGCGTCATGGTTACCACATTTCGGGCAAGTGAAGCCTTTGCTGGTGCACTCGAACTCACCGGTAAAGCCACACTCGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAG > minE/2902031‑2902161 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |