Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	668,428	C→A	T589K (ACG→AAG)	uup →	fused predicted transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	668,428	0	C	A	100.0%	204.0 / NA	61	T589K (ACG→AAG)	uup	fused predicted transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (54/7);  total (54/7)

CGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGATA  >  minE/668364‑668493                                                                 |                                                                  cgcgAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGt                                                             >  1:1075620/1‑71 (MQ=255) cgcgAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGAAGCAAGt                                                             >  1:2616379/1‑71 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                           <  1:1028921/66‑1 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                           <  1:1131701/66‑1 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                           <  1:344071/66‑1 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                           <  1:250483/66‑1 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                           <  1:2397930/66‑1 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                           <  1:1721787/66‑1 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAg                           <  1:1369546/66‑1 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGc                          >  1:3100626/1‑67 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGa                       >  1:70369/1‑70 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGa                       >  1:468017/1‑70 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGa                       >  1:3153086/1‑70 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGAc                      >  1:3094918/1‑71 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGAc                      >  1:1663546/1‑71 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGAc                      >  1:1794403/1‑71 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGAc                      >  1:524390/1‑71 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGAc                      >  1:2156805/1‑71 (MQ=255)                                        gAGGCGAAGCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGAc                      >  1:2173504/1‑71 (MQ=255)                                                gCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTGAGCCGCATGAGCAGACGCAAAAAg              >  1:4402/1‑71 (MQ=255)                                                gCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAg              >  1:1358436/1‑71 (MQ=255)                                                gCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAg              >  1:539094/1‑71 (MQ=255)                                                gCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAg              >  1:1300691/1‑71 (MQ=255)                                                gCTGGAAGCCCTACAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAg              >  1:2180292/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGTATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:2745741/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGt             >  1:3131392/1‑60 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:356921/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:878364/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:373767/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:753407/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:894850/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:406644/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:840566/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:759512/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:490393/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:602821/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:663218/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:697637/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:734422/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:2012337/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:1046326/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:1255128/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:126108/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:133836/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:1426287/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:1429906/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:146037/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:1497210/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:1631201/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:1649127/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:3114099/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:205278/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:2141882/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:2703015/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:2801508/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:2804331/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:2837248/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:2968509/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:2972389/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:304605/1‑71 (MQ=255)                                                            aCAGAAGCAAGTGGCGGATGCTTCCATCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGata  >  1:2723311/1‑71 (MQ=255)                                                                 |                                                                  CGCGAACTGGAGCAGCTACCGCAATTGCTCGAAGATCTGGAGGCGAAGCTGGAAGCCCTACAGACGCAAGTGGCGGATGCTTCCTTCTTCAGTCAGCCGCATGAGCAGACGCAAAAAGTGCTTGCTGATA  >  minE/668364‑668493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑