Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	692,900	T→C	A144A (GCT→GCC)	hyaD →	protein involved in processing of HyaA and HyaB proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	692,900	0	T	C	100.0%	300.6 / NA	80	A144A (GCT→GCC)	hyaD	protein involved in processing of HyaA and HyaB proteins
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (66/14);  total (66/14)

AACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGG  >  minE/692836‑692937                                                                 |                                      aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCTGCCGCGGaa                                 >  1:2851853/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:2784526/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:2286861/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:1994296/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:1972190/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:1955858/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:2353577/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:2413909/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:1821296/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:2458747/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:1742088/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:2605243/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:1640998/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:1633944/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:2671793/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:2871304/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:2903944/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:3245937/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:1300010/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:328373/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:36477/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:655154/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:752710/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:756495/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:869761/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGaa                                 >  1:962710/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGa                                  >  1:1507147/1‑70 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGa                                  >  1:824633/1‑70 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTACCCGCCGCGGaa                                 >  1:1773602/1‑71 (MQ=255) aaCCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAG‑AACTGCCCGCCGCGGaa                                 >  1:1037230/1‑70 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGTACTGGCACGGGAGCAACTGCCCGCCGCGGAACAggcggc                         >  1:3065666/1‑70 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGACCAGgcggcg                        >  1:1141055/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAggcggc                         >  1:355324/1‑70 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAggcggc                         >  1:2909736/1‑70 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:1534339/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:1353475/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:1290813/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:3297703/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:3085478/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:2977283/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:1204277/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:376800/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:1432559/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:473943/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:2828731/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:522452/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:58137/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:1723330/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:1806653/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:2391879/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:856452/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:2319588/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:1050893/1‑71 (MQ=255)          tGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGgcggcg                        >  1:2273621/1‑71 (MQ=255)                 cgacTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCTCGGAACAGGCGGCGCTGgcgc                 <  1:943585/71‑1 (MQ=255)                 cgacTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGgcgc                 <  1:3049811/71‑1 (MQ=255)                    cTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAgg                             <  1:2269165/56‑1 (MQ=255)                    cTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAgg                             <  1:1142782/56‑1 (MQ=255)                    cTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAgg                             <  1:70558/56‑1 (MQ=255)                    cTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAgg                             <  1:3178841/56‑1 (MQ=255)                    cTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAgg                             <  1:1482469/56‑1 (MQ=255)                    cTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAgg                             <  1:1630936/56‑1 (MQ=255)                           ggTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTtgctgc       <  1:962046/71‑1 (MQ=255)                           ggTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTtgctgc       <  1:2921726/71‑1 (MQ=255)                           ggTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTtgctgc       <  1:2077948/71‑1 (MQ=255)                           ggTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTtgctgc       <  1:2532468/71‑1 (MQ=255)                                 cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg  >  1:1028139/1‑70 (MQ=255)                                 cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg  >  1:928481/1‑70 (MQ=255)                                 cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg  >  1:1849402/1‑70 (MQ=255)                                 cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg  >  1:107678/1‑70 (MQ=255)                                 cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg  >  1:1086158/1‑70 (MQ=255)                                 cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg  >  1:753455/1‑70 (MQ=255)                                 cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg  >  1:2366011/1‑70 (MQ=255)                                 cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg  >  1:1832107/1‑70 (MQ=255)                                 cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg  >  1:2770777/1‑70 (MQ=255)                                 cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg  >  1:497458/1‑70 (MQ=255)                                 cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg  >  1:1393210/1‑70 (MQ=255)                                 cTGAGCGAACTGGCACGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTggg  >  1:3036865/1‑70 (MQ=255)                                                   gAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCTtgctgc       <  1:2239345/47‑1 (MQ=255)                                                      cAACGGCCCGCCGCGGAACAGGCGGCGCTGGCGCAGCGtgctgc       <  1:3187209/44‑1 (MQ=25)                                                                 |                                      AACCCGCAATGCTCGACGACTACGGCGGTAGCCTGAGCGAACTGGCACGGGAGCAACTGCCCGCTGCGGAACAGGCGGCGCTGGCGCAGCTTGCTGCGTGGG  >  minE/692836‑692937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑