Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 798,949 C→T M214I (ATG→ATA)  treA ← periplasmic trehalase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE798,9490CT100.0% 390.9 / NA 118M214I (ATG→ATA) treAperiplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (69/49);  total (69/49)

Only 100 of 118 total aligned reads displayed.

TGCGGCAGGTATTGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACCATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAAT  >  minE/798893‑799011
                                                        |                                                              
tGCGGCAGGTATTGCTTCAACGCGGCTTCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAaag                                                    >  1:699670/1‑69 (MQ=255)
tGCGGCAGGTATTGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAaag                                                    >  1:415120/1‑69 (MQ=255)
tGCGGCAGGTATTGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAaag                                                    >  1:411416/1‑69 (MQ=255)
tGCGGCAGGTATTGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAaag                                                    >  1:2626740/1‑69 (MQ=255)
tGCGGCAGGTATTGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAaag                                                    >  1:655452/1‑69 (MQ=255)
tGCGGCAGGTATTGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAaag                                                    >  1:1491325/1‑69 (MQ=255)
     cAGGTATTGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCg                                             >  1:2790672/1‑71 (MQ=255)
     cAGGTATTGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCg                                             >  1:2856934/1‑71 (MQ=255)
            tGCTTCAACGCGGGATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGc                                        >  1:1421410/1‑69 (MQ=255)
            tGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGc                                        >  1:3243919/1‑69 (MQ=255)
            tGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGc                                        >  1:2765044/1‑69 (MQ=255)
            tGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGc                                        >  1:2672096/1‑69 (MQ=255)
            tGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGc                                        >  1:1933311/1‑69 (MQ=255)
            tGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGc                                        >  1:2946417/1‑69 (MQ=255)
            tGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGc                                        >  1:1561578/1‑69 (MQ=255)
            tGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGc                                        >  1:2272190/1‑69 (MQ=255)
            tGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGc                                        >  1:1325229/1‑69 (MQ=255)
            tGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGc                                        >  1:730567/1‑69 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:132797/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:2706191/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:2677835/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:932859/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:593202/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:920106/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:3174384/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:2274749/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:901007/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:1826077/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:1809276/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:170093/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:3137287/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:890852/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:676281/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:3248592/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCgg                                  >  1:3268231/1‑71 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCg                                   >  1:2515892/1‑70 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCg                                   >  1:2257132/1‑70 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCg                                   >  1:1656107/1‑70 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCg                                   >  1:60053/1‑70 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCg                                   >  1:633262/1‑70 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCg                                   >  1:634699/1‑70 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCg                                   >  1:1533795/1‑70 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCg                                   >  1:1281814/1‑70 (MQ=255)
                tCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCg                                   >  1:1150152/1‑70 (MQ=255)
                       ggCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTaaa                            <  1:3288859/70‑1 (MQ=255)
                       ggCATCGCCTTCATGCTGCGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTaaa                            <  1:2333729/70‑1 (MQ=255)
                                     gctgcGCCAGTAACTCTACTATCAGGGCAAAGAAggg                                               >  1:20349/1‑37 (MQ=255)
                                     gctgcGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTg             >  1:2331872/1‑71 (MQ=255)
                                     gctgcGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTg             >  1:301506/1‑71 (MQ=255)
                                     gctgcGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTg             >  1:300412/1‑71 (MQ=255)
                                     gctgcGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTg             >  1:1848930/1‑71 (MQ=255)
                                     gctgcGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTg             >  1:19023/1‑71 (MQ=255)
                                     gctgcGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTg             >  1:1977522/1‑71 (MQ=255)
                                     gctgcGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTg             >  1:2028084/1‑71 (MQ=255)
                                     gctgcGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTg             >  1:1238094/1‑71 (MQ=255)
                                     gctgcGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTg             >  1:2081037/1‑71 (MQ=255)
                                     gctgcGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTg             >  1:2105341/1‑71 (MQ=255)
                                     gctgcGCCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTg             >  1:473741/1‑71 (MQ=255)
                                         cgcCAGTAACTCTACTATGAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGt         <  1:737173/71‑1 (MQ=255)
                                         cgcCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGt         <  1:2035197/71‑1 (MQ=255)
                                         cgcCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGt         <  1:491452/71‑1 (MQ=255)
                                         cgcCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGt         <  1:2841540/71‑1 (MQ=255)
                                         cgcCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGt         <  1:497546/71‑1 (MQ=255)
                                         cgcCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGt         <  1:1593272/71‑1 (MQ=255)
                                         cgcCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGt         <  1:1434029/71‑1 (MQ=255)
                                         cgcCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGt         <  1:222373/71‑1 (MQ=255)
                                          gcCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGt         <  1:193696/70‑1 (MQ=255)
                                          gcCAGTAACTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGt         <  1:2872334/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGTCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:1338558/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:959914/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:727056/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:37080/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:2583400/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:574706/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:1953714/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:1085651/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:1095295/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:1178091/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:1300382/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:136323/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:144312/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:1447612/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:1537389/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:1537714/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:1551597/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:18820/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:1894940/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:3230954/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:2024356/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:2367751/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:2401260/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:1075768/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:2590665/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:260534/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:2719721/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:2804850/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:2894074/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:2954664/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:3036733/70‑1 (MQ=255)
                                                 aCTCTACTATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAat  <  1:3058964/70‑1 (MQ=255)
                                                        |                                                              
TGCGGCAGGTATTGCTTCAACGCGGCATCGCCTTCATGCTGCGCCAGTAACTCTACCATCAGGGCAAAGAAGGGCGGTTGCGAGCGGCTTAAATAGTAACTGCGGTTGCCGTTGGGAAT  >  minE/798893‑799011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑