BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	52,517	T→C	E757E (GAA→GAG)	imp ←	exported protein required for envelope biosynthesis and integrity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	52,517	0	T	C	100.0%	480.9 / NA	128	E757E (GAA→GAG)	imp	exported protein required for envelope biosynthesis and integrity
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (56/72); total (56/72)

Only 100 of 128 total aligned reads displayed.

TACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCC  >  minE/52449‑52571

tACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                      <  1:760365/71‑1 (MQ=255)

tACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                      <  1:2484742/71‑1 (MQ=255)

tACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                      <  1:2766615/71‑1 (MQ=255)

tACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                      <  1:155786/71‑1 (MQ=255)

tACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                      <  1:3295892/71‑1 (MQ=255)

tACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                      <  1:202573/71‑1 (MQ=255)

tACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                      <  1:848734/71‑1 (MQ=255)

tACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                      <  1:1891449/71‑1 (MQ=255)

tACGGCAGAATGTTCGAACGCAGCAGCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                      <  1:945651/71‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:2094707/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:2198241/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:2641997/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:2005792/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:226343/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:2281405/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:2291869/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:2408153/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:2495532/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:2621694/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:1035347/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:2788887/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:3151304/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:3184474/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:763889/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:787412/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:825274/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:866995/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:957356/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:1384694/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:1427269/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:1482257/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:1667206/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:1093145/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:1188259/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:1769381/70‑1 (MQ=255)

  cGGCAGAATGTTCGAACGCAGCATCTCTTGCGTAACCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:2164194/70‑1 (MQ=255)

   ggCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCg                                                     <  1:853794/69‑1 (MQ=255)

               gAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTc                                                      <  1:357534/56‑1 (MQ=255)

                             ttGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCa                             <  1:2027121/67‑1 (MQ=255)

                             ttGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCa                             <  1:209936/67‑1 (MQ=255)

                             ttGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCa                             <  1:3003725/67‑1 (MQ=255)

                             ttGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCa                             <  1:2524614/67‑1 (MQ=255)

                                    cccAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtg                  <  1:1811361/71‑1 (MQ=255)

                                    cccAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtg                  <  1:47102/71‑1 (MQ=255)

                                    cccAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtg                  <  1:2820789/71‑1 (MQ=255)

                                    cccAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtg                  <  1:2648083/71‑1 (MQ=255)

                                    cccAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtg                  <  1:335786/71‑1 (MQ=255)

                                    cccAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtg                  <  1:2600865/71‑1 (MQ=255)

                                    cccAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCAtg                  <  1:292498/71‑1 (MQ=255)

                                      cAGACCGTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCa                             <  1:2903162/58‑1 (MQ=255)

                                            gaaGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGttt            >  1:1829873/3‑69 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCAtata                         >  1:78517/1‑56 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTa           >  1:3186068/1‑70 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:3001341/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:3060140/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:930082/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:1017793/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:282919/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:2881935/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:926216/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:1355292/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:543363/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:2738215/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:1836704/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:1874085/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:1704728/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:2186724/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:2230344/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:1530773/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:1504600/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:2752701/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:2546741/1‑71 (MQ=255)

                                            gTAGTTGGAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTAt          >  1:2735672/1‑71 (MQ=255)

                                                  ggAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGAt                                       >  1:1279350/1‑36 (MQ=255)

                                                  ggAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCg                      >  1:979798/1‑53 (MQ=255)

                                                  ggAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCg                      >  1:10360/1‑53 (MQ=255)

                                                  ggAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCg                      >  1:941323/1‑53 (MQ=255)

                                                  ggAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCg                      >  1:1965908/1‑53 (MQ=255)

                                                  ggAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCg                      >  1:835721/1‑53 (MQ=255)

                                                  ggAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCg                      >  1:116892/1‑53 (MQ=255)

                                                  ggAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCg                      >  1:1777251/1‑53 (MQ=255)

                                                  ggAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCg                      >  1:1204205/1‑53 (MQ=255)

                                                  ggAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCg                      >  1:1273482/1‑53 (MQ=255)

                                                  ggAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCg                      >  1:433182/1‑53 (MQ=255)

                                                  ggAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCg                      >  1:1310823/1‑53 (MQ=255)

                                                  ggAGCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCg                      >  1:1538190/1‑53 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCa                    <  1:1931309/52‑1 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCa                    <  1:153074/52‑1 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCa                    <  1:308110/52‑1 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCa                    <  1:1351187/52‑1 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCa                    <  1:93391/52‑1 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCa                    <  1:1056990/52‑1 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCa                    <  1:759547/52‑1 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCa                    <  1:149036/52‑1 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCa                    <  1:572274/52‑1 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATcc  >  1:1812501/1‑70 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATcc  >  1:1871843/1‑70 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATcc  >  1:1535170/1‑70 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATcc  >  1:355593/1‑70 (MQ=255)

                                                     gCTCAGGCCGCGAAGCTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATcc  >  1:2030644/1‑70 (MQ=255)

TACGGCAGAATGTTCGAACGCAGCATCTCTTGCGTACCCAGACCGTAGTTGGAGCTCAGGCCGCGAAGTTCGATGTTAAAGCCGATTGCGTTGTCATATACCGCATGTTGTTTATCGTTATCC  >  minE/52449‑52571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`