Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,013,547	G→A	R245C (CGC→TGC)	fumC ←	fumarate hydratase (fumarase C), aerobic Class II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,013,547	0	G	A	100.0%	64.9 / NA	20	R245C (CGC→TGC)	fumC	fumarate hydratase (fumarase C), aerobic Class II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/20);  total (0/20)

CACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCC  >  minE/1013514‑1013584                                  |                                      cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTGGTCCAGTAccc  <  1:1405031/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:2241226/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:953116/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:798697/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:378921/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:3291755/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:2925408/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:2738213/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:2455378/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:2447530/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:22536/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:1002924/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:2113539/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:2107403/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:1995162/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:1924547/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:1576076/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:1116675/71‑1 (MQ=255) cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:109482/71‑1 (MQ=255)  acaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc  <  1:180556/70‑1 (MQ=255)                                  |                                      CACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCC  >  minE/1013514‑1013584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑