BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,081,858	T→C	N104S (AAC→AGC)	ydhY ←	predicted 4Fe‑4S ferridoxin‑type protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,081,858	0	T	C	100.0%	229.5 / NA	62	N104S (AAC→AGC)	ydhY	predicted 4Fe‑4S ferridoxin‑type protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/41); total (21/41)

TCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTC  >  minE/1081807‑1081908

tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGATCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:2513545/71‑1 (MQ=255)

tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:345196/71‑1 (MQ=255)

tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:1070117/71‑1 (MQ=255)

tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:2919989/71‑1 (MQ=255)

tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:2251954/71‑1 (MQ=255)

tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:2167212/71‑1 (MQ=255)

tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:2152255/71‑1 (MQ=255)

tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:1881531/71‑1 (MQ=255)

tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:1778080/71‑1 (MQ=255)

tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:1539307/71‑1 (MQ=255)

tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:1484460/71‑1 (MQ=255)

gccGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:1291372/70‑1 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGaaa                                      >  1:1051662/1‑64 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 >  1:1483874/1‑69 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 >  1:192928/1‑69 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:1072571/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:966994/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:894002/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:488858/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:3060318/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2948180/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:1072063/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2858505/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2689699/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2678820/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2553828/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2472351/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:1910981/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:1793062/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:1907098/1‑71 (MQ=255)

  cgcgGTATAGTTGAGATCACCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGc                               >  1:2391661/1‑71 (MQ=255)

      gTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATg                           <  1:3095931/71‑1 (MQ=255)

      gTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATg                           <  1:407877/71‑1 (MQ=255)

        ataGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGa                         >  1:1551496/1‑71 (MQ=255)

         taGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGAt                        >  1:1164925/1‑71 (MQ=255)

            ttGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:2371542/59‑1 (MQ=255)

                     ccATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                 <  1:804880/50‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACTGGAGAAGAATGTc  <  1:859298/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:832117/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1715129/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1681653/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:870409/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1605423/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:4047/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:963139/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1017770/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:3252731/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:987165/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:3091487/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1692828/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1750451/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1413159/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1166546/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:2656853/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1894925/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1486650/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:2419255/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:2290872/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:1073310/71‑1 (MQ=255)

                               cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:2087898/71‑1 (MQ=255)

                                 ccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATTGATTTTGATACGGGAGAAGAATGTc  <  1:1087568/69‑1 (MQ=255)

                                 ccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc  <  1:884064/69‑1 (MQ=255)

TCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTC  >  minE/1081807‑1081908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`