Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,151,211	A→G	V2V (GTA→GTG)	ydjQ →	endonuclease of nucleotide excision repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,151,211	0	A	G	100.0%	177.2 / NA	48	V2V (GTA→GTG)	ydjQ	endonuclease of nucleotide excision repair
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/32);  total (16/32)

CTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAA  >  minE/1151145‑1151254                                                                   |                                            ctctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAATCAGCATTCGGAGTCAACAGTGGTGCgg                                          >  1:919256/1‑70 (MQ=255) ctctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCgg                                          >  1:922634/1‑70 (MQ=255) ctctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCgg                                          >  1:3194343/1‑70 (MQ=255) ctctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGc                                         >  1:1088808/1‑71 (MQ=255) ctctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGc                                         >  1:26539/1‑71 (MQ=255) ctctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGc                                         >  1:1988218/1‑71 (MQ=255) ctctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGc                                         >  1:1894326/1‑71 (MQ=255) ctctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGc                                         >  1:1751951/1‑71 (MQ=255) ctctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGc                                         >  1:1047926/1‑71 (MQ=255) ctctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGc                                         >  1:124461/1‑71 (MQ=255) ctctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGc                                         >  1:1519526/1‑71 (MQ=255) ctctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGc                                         >  1:1314358/1‑71 (MQ=255)                          ttACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGaa                  <  1:3201192/69‑1 (MQ=255)                          ttACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGaa                  <  1:2541009/69‑1 (MQ=255)                                        taACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCaa  <  1:1694770/71‑1 (MQ=255)                                        taACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCaa  <  1:2781150/71‑1 (MQ=255)                                        taACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCaa  <  1:275130/71‑1 (MQ=255)                                        taACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCaa  <  1:1579137/71‑1 (MQ=255)                                        taACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCaa  <  1:1137922/71‑1 (MQ=255)                                        taACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCaa  <  1:2570039/71‑1 (MQ=255)                                        taACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCaa  <  1:2551212/71‑1 (MQ=255)                                        taACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCaa  <  1:3274939/71‑1 (MQ=255)                                        taACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCaa  <  1:372915/71‑1 (MQ=255)                                        taACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCaa  <  1:1370947/71‑1 (MQ=255)                                        taACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCaa  <  1:1698446/71‑1 (MQ=255)                                        taACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCaa  <  1:16917/71‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:324499/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:500165/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:566736/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:734960/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:743005/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:754577/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:2681895/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:2701846/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:2454655/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:2251192/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:2211900/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:220555/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:2032964/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:1856737/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:1429735/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:15813/57‑1 (MQ=255)                                          aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:1527745/57‑1 (MQ=255)                                            cAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTtc                               >  1:390733/1‑38 (MQ=255)                                            cAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGaa            >  1:3250503/1‑57 (MQ=255)                                            cAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGaa            >  1:423766/1‑57 (MQ=255)                                            cAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGaa            >  1:8323/1‑57 (MQ=255)                                                             aGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg              <  1:1384447/38‑1 (MQ=255)                                                                   |                                            CTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAA  >  minE/1151145‑1151254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑