Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,193,556	T→C	Y36C (TAC→TGC)	fadD ←	acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,193,556	0	T	C	100.0%	104.9 / NA	29	Y36C (TAC→TGC)	fadD	acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/18);  total (11/18)

AGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGG  >  minE/1193502‑1193623                                                       |                                                                    aGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTg                                                     <  1:2858012/71‑1 (MQ=255) aGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTg                                                     <  1:2578383/71‑1 (MQ=255) aGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTg                                                     <  1:2327221/71‑1 (MQ=255) aGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTg                                                     <  1:535664/71‑1 (MQ=255) aGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTg                                                     <  1:1836413/71‑1 (MQ=255)  gCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTg                                                     >  1:1238936/1‑70 (MQ=255)  gCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGc                                                    >  1:3187696/1‑71 (MQ=255)  gCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGc                                                    >  1:2837223/1‑71 (MQ=255)  gCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGc                                                    >  1:2922856/1‑71 (MQ=255)  gCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGc                                                    >  1:3060804/1‑71 (MQ=255)  gCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGc                                                    >  1:223680/1‑71 (MQ=255)  gCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGc                                                    >  1:1843976/1‑71 (MQ=255)  gCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGc                                                    >  1:840202/1‑71 (MQ=255)  gCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGc                                                    >  1:1660927/1‑71 (MQ=255)  gCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGc                                                    >  1:788607/1‑71 (MQ=255)  gCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGc                                                    >  1:1441081/1‑71 (MQ=255)                        ccccATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGCTCAAACATATCTACCAgaga                                <  1:1853560/69‑1 (MQ=255)                        ccccATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGCTCAAACATATCTACCAgaga                                <  1:2190275/69‑1 (MQ=255)                        ccccATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGCTCAAACATATCTACCAgaga                                <  1:2231334/69‑1 (MQ=255)                        ccccATATTCACAAACGCAGGTTGATCGGCGCAGCGCGCGACCGACTGCTCAAACATATCTACCAgaga                                <  1:2834586/69‑1 (MQ=255)                                            gTTGATCGGCGCAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGAtc          <  1:10090/71‑1 (MQ=255)                                            gTTGATCGGCGCAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGAtc          <  1:2753884/71‑1 (MQ=255)                                            gTTGATCGGCGCAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGAtc          <  1:2931995/71‑1 (MQ=255)                                            gTTGATCGGCGCAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGAtc          <  1:2625017/71‑1 (MQ=255)                                            gTTGATCGGCGCAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGAtc          <  1:3055284/71‑1 (MQ=255)                                            gTTGATCGGCGCAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGAtc          <  1:2315171/71‑1 (MQ=255)                                            gTTGATCGGCGCAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGAtc          <  1:1742527/71‑1 (MQ=255)                                            gTTGATCGGCGCAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGAtc          <  1:3189905/71‑1 (MQ=255)                                            gTTGATCGGCGCAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGGCAGGGTTGAtc          <  1:1306294/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGTTTGATCTCCGTCgg  <  1:1045141/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:2899364/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:2764129/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:268878/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:2318326/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:2254977/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:2231241/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:2210985/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:3260193/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:371947/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:1791034/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:561684/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:637553/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:1488464/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:1123512/71‑1 (MQ=255)                                                    gcgcAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCgg  <  1:931275/71‑1 (MQ=255)                                                       |                                                                    AGCTTGCGGAAGGTCATTACCTCCCCCATATTCACAAACGCAGGTTGATCGGCGTAGCGCGCGACCGACTGCTCAAACATATCTACCAGAGATTGATAACGGTCAGGGTTGATCTCCGTCGG  >  minE/1193502‑1193623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑