Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,231,014	C→T	G581D (GGT→GAT)	ptrB ←	protease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,231,014	0	C	T	100.0%	52.3 / NA	17	G581D (GGT→GAT)	ptrB	protease II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/15);  total (2/15)

CGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTACCCCACTCTTCAAACTCACCAGTG  >  minE/1230955‑1231037                                                            |                        cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:2627594/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:943163/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:845001/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:789755/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:604337/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:374622/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:3016896/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:2661359/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:1034911/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:2575441/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:2112375/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:1901511/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:154566/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:1343157/71‑1 (MQ=255) cGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCa              <  1:1278110/71‑1 (MQ=255)                        ttCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCAAACTCACCAGTg  >  1:1897227/1‑60 (MQ=255)                        ttCATGTACTCGTAATATTGCGGATCCTGCGGGTTATCCCACTCTTCAAACTCACCAGTg  >  1:733922/1‑60 (MQ=255)                                                            |                        CGTTGTCATACGGGCTGTAGCTTTTCATGTACTCGTAATATTGCGGATCCTGCGGGTTACCCCACTCTTCAAACTCACCAGTG  >  minE/1230955‑1231037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑