Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,252,050	T→C	K6K (AAA→AAG)	yebC ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,252,050	0	T	C	100.0%	16.9 / NA	6	K6K (AAA→AAG)	yebC	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/0);  total (6/0)

ATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAA  >  minE/1251982‑1252109                                                                     |                                                            aTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCActt                                                           <  1:1450422/71‑1 (MQ=255) aTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCActt                                                           <  1:2705619/71‑1 (MQ=255) aTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCActt                                                           <  1:2766637/71‑1 (MQ=255) aTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCActt                                                           <  1:2922628/71‑1 (MQ=255) aTGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCActt                                                           <  1:483085/71‑1 (MQ=255)  tGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCActt                                                           <  1:1132461/70‑1 (MQ=255)                                                          gtgtTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCcctgcc                   >  1:2640538/1‑54 (MQ=255)                                                          gtgtTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTaa  >  1:2566538/1‑71 (MQ=255)                                                          gtgtTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTaa  >  1:2870659/1‑71 (MQ=255)                                                          gtgtTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTaa  >  1:438794/1‑71 (MQ=255)                                                          gtgtTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTaa  >  1:536969/1‑71 (MQ=255)                                                          gtgtTGGCCCACTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTa   >  1:3002338/1‑70 (MQ=255)                                                                     |                                                            ATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGCAGCGTTAA  >  minE/1251982‑1252109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑