Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,253,716	C→T	A241A (GCG→GCA)	aspS ←	aspartyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,253,716	0	C	T	100.0%	225.6 / NA	68	A241A (GCG→GCA)	aspS	aspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/68);  total (0/68)

ACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAG  >  minE/1253699‑1253771                  |                                                        aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2358100/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:214785/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:688922/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2178864/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2179740/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2216726/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2235298/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2248308/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2252183/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2258783/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:1108206/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:229236/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2177009/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2417922/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2435919/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2534777/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:520159/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2776570/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:450336/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2963369/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:3143756/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:3202595/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:3262488/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2120444/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:984436/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:1183150/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:1198330/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:852358/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:1286745/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:835883/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:1397462/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:791521/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:1651151/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:1729220/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2051378/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:753785/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2104988/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:1750394/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:1907269/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:1865461/71‑1 (MQ=255) aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:176472/71‑1 (MQ=255)  cTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:1531026/70‑1 (MQ=255)  cTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:759376/70‑1 (MQ=255)  cTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:44337/70‑1 (MQ=255)  cTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2105248/70‑1 (MQ=255)  cTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTACACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:2326343/70‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:558792/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:785429/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:451309/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:2273079/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:338941/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:3266796/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:964740/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:3112605/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:307589/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:3040046/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:2917911/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:278606/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:2745580/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:2562447/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:2166332/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:2139525/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:1958829/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:1558382/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:1334032/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:1271641/69‑1 (MQ=255)     cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:1111322/69‑1 (MQ=255)      aCGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg  <  1:1479947/68‑1 (MQ=255)               cggtgCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTc    <  1:1179496/57‑1 (MQ=255)                  |                                                        ACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAG  >  minE/1253699‑1253771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑