| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 91,462 | T→C | S56P (TCT→CCT) | lpxC → | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 91,462 | 0 | T | C | 100.0% | 13.0 / NA | 5 | S56P (TCT→CCT) | lpxC | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (5/0); total (5/0) | |||||||||||
CAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCG > minE/91458‑91525 | cAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAAcc > 1:1127215/1‑67 (MQ=255)cAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAAcc > 1:568334/1‑67 (MQ=255)cAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCg > 1:2164123/1‑68 (MQ=255)cAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCg > 1:3251487/1‑68 (MQ=255)cAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCg > 1:8690/1‑68 (MQ=255) | CAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCG > minE/91458‑91525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |