| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,325,522 | T→G | K71T (AAA→ACA) | wcaL ← | predicted glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,325,522 | 0 | T | G | 100.0% | 16.9 / NA | 6 | K71T (AAA→ACA) | wcaL | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (0/6); total (0/6) | |||||||||||
CCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCG > minE/1325489‑1325559 | ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg < 1:1015066/71‑1 (MQ=255)ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg < 1:1510532/71‑1 (MQ=255)ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg < 1:1794849/71‑1 (MQ=255)ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg < 1:338216/71‑1 (MQ=255)ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg < 1:394378/71‑1 (MQ=255)ccGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTGTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg < 1:540160/71‑1 (MQ=255) | CCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCG > minE/1325489‑1325559 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |