Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,330,586	A→G	Y334H (TAC→CAC)	cpsG ←	phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,330,586	0	A	G	100.0%	139.4 / NA	38	Y334H (TAC→CAC)	cpsG	phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/17);  total (21/17)

CAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCAT  >  minE/1330524‑1330634                                                               |                                                 cAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCCCGGAAGTGATGGTggg                                          >  1:973188/1‑71 (MQ=255) cAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTggg                                          >  1:969445/1‑71 (MQ=255) cAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTggg                                          >  1:90129/1‑71 (MQ=255) cAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTggg                                          >  1:894981/1‑71 (MQ=255) cAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTggg                                          >  1:2800441/1‑71 (MQ=255) cAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTggg                                          >  1:240913/1‑71 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAtgg                                              >  1:993288/1‑66 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTggg                                          >  1:2702857/1‑70 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgc                                         >  1:77589/1‑71 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgc                                         >  1:3142448/1‑71 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgc                                         >  1:3054195/1‑71 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgc                                         >  1:2949012/1‑71 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgc                                         >  1:560797/1‑71 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgc                                         >  1:569452/1‑71 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgc                                         >  1:611653/1‑71 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgc                                         >  1:2565806/1‑71 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgc                                         >  1:2478762/1‑71 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgc                                         >  1:678295/1‑71 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgc                                         >  1:2292743/1‑71 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgc                                         >  1:1581848/1‑71 (MQ=255)  aGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGATGGTGGgc                                         >  1:1172480/1‑71 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:554516/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:997659/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:1011658/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:391394/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:3203976/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:2732427/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:2639639/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:2625933/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:243815/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:2437585/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:2100972/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:2029141/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:1996456/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:1368459/36‑1 (MQ=255)                              tGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTGAt                                                <  1:1071017/36‑1 (MQ=255)                                         cAGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCAt  <  1:607299/71‑1 (MQ=255)                                          aGTAAGCGAAATCACGGAAGTGATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCAt  <  1:1425671/70‑1 (MQ=255)                                                               |                                                 CAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCAT  >  minE/1330524‑1330634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑