Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,439,412	G→A	A297V (GCT→GTT)	ompC ←	outer membrane porin protein C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,439,412	0	G	A	100.0%	299.1 / NA	100	A297V (GCT→GTT)	ompC	outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (75/25);  total (75/25)

TCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTG  >  minE/1439361‑1439477                                                    |                                                                  tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGggac                                                           >  1:659814/1‑60 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:952407/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:340329/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:269659/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:1417041/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:2479135/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:917553/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:1467743/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:366748/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:1294893/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:129087/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:700935/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:125740/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:1244023/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:2990185/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:1777125/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:3135181/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:1875085/1‑71 (MQ=255)         gtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCg                                         >  1:764486/1‑70 (MQ=255)         gtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCg                                         >  1:1073621/1‑70 (MQ=255)            gtAGCCACGCCCCAGGTTTTTACCTTTAGCCTGCAGGTAAACCAGGGACGGACGCAGAcc                                                >  1:3182834/1‑60 (MQ=255)            gtAGCCACGACCCGGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:943196/1‑71 (MQ=39)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACTGACGCAGACCGAAGTCGAACt                                     >  1:2811762/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAAc                                      >  1:1721915/1‑70 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:2658259/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:2806866/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:409870/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:1994889/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:408963/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:2154212/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:3030483/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:2234016/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:2273388/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:518298/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:2833621/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:231186/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:2338527/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:2369253/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:2474586/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:970259/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:1833235/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:1150311/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:1181403/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:1265240/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:949442/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:1419467/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:1445815/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:1681105/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:153364/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACt                                     >  1:1590446/1‑71 (MQ=255)            gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGCACGCAGACCGAAGTCGAACt                                     >  1:510954/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTTGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:1868724/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:709601/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:3175456/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:317821/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:2066085/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:1204760/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:2951971/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:2934726/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:2079705/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:868928/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:2760729/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:369082/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:844477/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:261357/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:1771206/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:1446743/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:1517451/1‑71 (MQ=255)                 cACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTAc                                >  1:16085/1‑71 (MQ=255)                            tttttACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGc                     >  1:583964/1‑71 (MQ=255)                            tttttACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGc                     >  1:990740/1‑71 (MQ=255)                            tttttACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGc                     >  1:3003466/1‑71 (MQ=255)                            tttttACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGc                     >  1:1420225/1‑71 (MQ=255)                            tttttACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGc                     >  1:2456726/1‑71 (MQ=255)                            tttttACCTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGc                     >  1:2229252/1‑71 (MQ=255)                                  gcTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:1806742/69‑1 (MQ=255)                                  ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:183599/70‑1 (MQ=255)                                  ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:788677/70‑1 (MQ=255)                                  ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:164196/70‑1 (MQ=255)                                  ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:1415056/70‑1 (MQ=255)                                  ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:1371317/70‑1 (MQ=255)                                  ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:345662/70‑1 (MQ=255)                                  ccTTTAGACTGCAGGTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGa                <  1:2992282/70‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:133224/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:1089789/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:1286089/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:1098797/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:3166071/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:1439006/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:1608719/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:1620319/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:68316/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:601778/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:1796628/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:578462/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:512497/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:1993088/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:2059266/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg  <  1:2589951/71‑1 (MQ=255)                                               ggTAAACCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTCTg  <  1:1400485/71‑1 (MQ=255)                                                    |                                                                  TCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTG  >  minE/1439361‑1439477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑