| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,509,579 | G→A | A42T (GCA→ACA) | ackA → | acetate kinase A and propionate kinase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,509,579 | 0 | G | A | 98.2% | 179.5 / NA | 54 | A42T (GCA→ACA) | ackA | acetate kinase A and propionate kinase 2 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/44); minor base T (0/1); total (9/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAATGGAAAATGGACGGCAATAAACAGG > minE/1509533‑1509615 | tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:2987100/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:2229569/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:2448167/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:2729493/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:2803530/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:2839124/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:2870527/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:2874151/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:2936150/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:2541955/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:3006223/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:3187841/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:3241848/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:3256744/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:335435/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:590581/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:715654/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:951071/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:1847003/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:1188479/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:1323366/71‑1 (MQ=255)tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg < 1:1892103/71‑1 (MQ=255) gAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACgg > 1:1561762/1‑71 (MQ=255) gAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACgg > 1:706235/1‑71 (MQ=255) gAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACgg > 1:1845325/1‑71 (MQ=255) gAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACgg > 1:2406087/1‑71 (MQ=255) gAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACgg > 1:3193785/1‑71 (MQ=255) gAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACgg > 1:1973468/1‑71 (MQ=255) gAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACgg > 1:1957054/1‑71 (MQ=255) agagTACCTTTCTGGTTTAGTCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:1057904/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:125231/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:1227951/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:554991/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:600883/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:6464/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:166492/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:3141853/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:3111968/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:3100891/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:2181450/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:1836175/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:185245/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:2860048/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:1016084/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:1988078/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:2440701/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:2312891/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:2282289/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:2088408/71‑1 (MQ=255) agagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:2198454/71‑1 (MQ=255) gagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa < 1:375709/70‑1 (MQ=255) cTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAgg > 1:2474456/1‑69 (MQ=255) cTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAgg > 1:558732/1‑69 (MQ=255) cACCTGCCCGAATCACGTATCAAATGGAAAATGGACg < 1:10959/37‑1 (MQ=255) | TGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAATGGAAAATGGACGGCAATAAACAGG > minE/1509533‑1509615 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |