Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	144,272	C→A	G194V (GGC→GTC)	yadB ←	glutamyl‑Q tRNA(Asp) synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	144,272	0	C	A	100.0%	201.7 / NA	62	G194V (GGC→GTC)	yadB	glutamyl‑Q tRNA(Asp) synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/34);  total (28/34)

AGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACG  >  minE/144212‑144327                                                             |                                                        aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCGACGCACTATTTCTGTAACGACCTGGAAATg                                               <  1:887551/71‑1 (MQ=255) aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                                               <  1:42091/71‑1 (MQ=255) aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                                               <  1:2672921/71‑1 (MQ=255) aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                                               <  1:175674/71‑1 (MQ=255) aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                                               <  1:1642691/71‑1 (MQ=255) aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                                               <  1:2749590/71‑1 (MQ=255) aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                                               <  1:52396/71‑1 (MQ=255) aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                                               <  1:2977972/71‑1 (MQ=255) aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                                               <  1:1363657/71‑1 (MQ=255) aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                                               <  1:927719/71‑1 (MQ=255) aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                                               <  1:45123/71‑1 (MQ=255) aGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCAACGCACTATTTCTGTAACGACCTGGAAATg                                               <  1:3193790/71‑1 (MQ=255)  gCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTTTAACGACCTGGAAATg                                               <  1:2780259/70‑1 (MQ=255)  gCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATg                                               <  1:550922/70‑1 (MQ=255)                               agctcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACAACAACAGCCAGGTTGTAGGc                 >  1:1451897/4‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGc                             >  1:3109151/1‑59 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:2661810/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:449216/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:2679469/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:2730373/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:3240484/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:2739119/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:3218239/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:2761134/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:546673/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:2980254/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:1941898/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:1042055/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:1129431/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:1596993/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:1593171/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:661771/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:1525306/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:1507169/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:1152976/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:2504438/1‑71 (MQ=255)                               agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                 >  1:780352/1‑71 (MQ=255)                                             cACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATc     >  1:2360992/1‑69 (MQ=255)                                             cACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCAc   >  1:1459085/1‑71 (MQ=255)                                             cACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCAc   >  1:133266/1‑71 (MQ=255)                                             cACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCAc   >  1:1495820/1‑71 (MQ=255)                                             cACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCAAGGTTGTAGGCGAACAACCCATCAc   >  1:3069230/1‑71 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:494267/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:742955/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:65736/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:932043/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:582393/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:994442/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:377644/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:3187850/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:2968546/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:2813275/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:2107172/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:1911209/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:1843651/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:1564819/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:143025/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:1366720/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:1285235/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:1191457/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:111373/71‑1 (MQ=255)                                              aCTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACg  <  1:1101203/71‑1 (MQ=255)                                                             |                                                        AGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACG  >  minE/144212‑144327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑