Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,536,834	C→T	M1I (ATG→ATA) †	yfcR ←	predicted fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,536,834	0	C	T	100.0%	148.4 / NA	45	M1I (ATG→ATA) †	yfcR	predicted fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/37);  total (8/37)

CTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGC  >  minE/1536769‑1536899                                                                  |                                                                  cTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACT‑CCCCCGTTATCAGc                                                             <  1:2439766/71‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:2196259/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:41087/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:3217301/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:3096701/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:754333/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:3051624/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:2849963/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:2775693/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:2445621/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:2381420/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:2348825/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:2041817/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:1803448/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:150822/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:1014924/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:1262454/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:1357380/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:1362038/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:1411545/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:1476467/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:1492205/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:1716953/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:1607662/69‑1 (MQ=255)    cAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:1611409/69‑1 (MQ=255)      gACACTGCATACCTGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGc                                                             <  1:2763895/67‑1 (MQ=255)                 ccAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctgc                                               <  1:1631632/70‑1 (MQ=255)                 ccAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctgc                                               <  1:954526/70‑1 (MQ=255)                 ccAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctgc                                               <  1:780075/70‑1 (MQ=255)                 ccAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctgc                                               <  1:2249051/70‑1 (MQ=255)                 ccAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctgc                                               <  1:1638803/70‑1 (MQ=255)                 ccAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctgc                                               <  1:8647/70‑1 (MQ=255)                 ccAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctgc                                               <  1:18978/70‑1 (MQ=255)                 ccAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctgc                                               <  1:1648737/70‑1 (MQ=255)                         ccTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATc                                       >  1:2822673/1‑70 (MQ=255)                         ccTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATc                                       >  1:194168/1‑70 (MQ=255)                         ccTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATc                                       >  1:669859/1‑70 (MQ=255)                                                       aCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTc                                          >  1:804908/1‑37 (MQ=255)                                                       aCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCa                                         >  1:3196214/1‑38 (MQ=255)                                                       aCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCa                                         >  1:3172510/1‑38 (MQ=255)                                                       aCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCa                                         >  1:3061585/1‑38 (MQ=255)                                                       aCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCa                                         >  1:232781/1‑38 (MQ=255)                                                             cccGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGGTGTTGCCATTGCACTGGAAGAACAGc  <  1:1225111/71‑1 (MQ=255)                                                             cccGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGc  <  1:2333829/71‑1 (MQ=255)                                                               cGTTATCAGCCTTTGCGCCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGc  <  1:471733/69‑1 (MQ=255)                                                                  |                                                                  CTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGC  >  minE/1536769‑1536899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑