Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,591,415	G→T	K203N (AAG→AAT)	yfeS →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,591,415	0	G	T	100.0%	187.6 / NA	55	K203N (AAG→AAT)	yfeS	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (47/8);  total (47/8)

TGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAG  >  minE/1591364‑1591481                                                    |                                                                   tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTa                                                  >  1:2056488/1‑70 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:1070524/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:3062829/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:3027001/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:32390/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:261338/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:26124/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:2612050/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:100184/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:2600549/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:2596828/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:2550440/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:3287178/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:2453492/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:672035/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:2354066/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:2145158/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:1074524/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:932988/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:1229189/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:809992/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:1560142/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:751653/1‑71 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:1932036/1‑71 (MQ=255) tGCATTAGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg                                                 >  1:2731227/1‑71 (MQ=255)                                        tAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAg                                      >  1:2502655/1‑43 (MQ=255)                                        tAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGt           >  1:703056/1‑70 (MQ=255)                                        tAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGt           >  1:1940522/1‑70 (MQ=255)                                        tAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGt           >  1:690109/1‑70 (MQ=255)                                        tAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGt           >  1:2973200/1‑70 (MQ=255)                                        tAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGt           >  1:2170685/1‑70 (MQ=255)                                        tAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGt           >  1:3140245/1‑70 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATgg              >  1:810914/1‑65 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGa         >  1:3248899/1‑70 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGAc        >  1:3252045/1‑71 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGAc        >  1:696782/1‑71 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGAc        >  1:2611287/1‑71 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGAc        >  1:3183655/1‑71 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGAc        >  1:3155532/1‑71 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGAc        >  1:2582863/1‑71 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGAc        >  1:117090/1‑71 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGAc        >  1:1288668/1‑71 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGAc        >  1:2363740/1‑71 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGAc        >  1:2085784/1‑71 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGAc        >  1:2079471/1‑71 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGAc        >  1:1713098/1‑71 (MQ=255)                                          aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGAc        >  1:1411702/1‑71 (MQ=255)                                                aaaaTATGGGACTCGATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAg  <  1:549728/71‑1 (MQ=255)                                                aaaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAg  <  1:99768/71‑1 (MQ=255)                                                aaaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAg  <  1:996765/71‑1 (MQ=255)                                                aaaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAg  <  1:1609476/71‑1 (MQ=255)                                                aaaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAg  <  1:6652/71‑1 (MQ=255)                                                aaaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAg  <  1:2469795/71‑1 (MQ=255)                                                aaaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAg  <  1:2608054/71‑1 (MQ=255)                                                aaaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAg  <  1:2846393/71‑1 (MQ=255)                                                    |                                                                   TGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAG  >  minE/1591364‑1591481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑