Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,643,860	A→G	R9R (CGT→CGC)	purC ←	phosphoribosylaminoimidazole‑succinocarboxamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,643,860	0	A	G	100.0%	365.5 / NA	98	R9R (CGT→CGC)	purC	phosphoribosylaminoimidazole‑succinocarboxamide synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/76);  total (22/76)

GAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATC  >  minE/1643803‑1643924                                                          |                                                                 gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:2056612/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:1641007/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:182070/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:1828784/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:582886/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:1852865/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:144859/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:200250/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:3194494/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:3132412/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:1289362/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:2131158/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:1228348/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:1213126/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:3107964/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:2199350/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:1135512/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:1126610/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:2550966/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:947557/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATAAGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:2608992/1‑71 (MQ=255) gAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGATGTATACGGTTTTCGCTTTACCGCGATACAACTCAg                                                     >  1:813257/1‑71 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTTCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1375913/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2897430/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:83723/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:3020427/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2962815/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:847766/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2951098/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2946327/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2939806/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:3120814/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2893653/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2848022/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:939890/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2844464/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2820968/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2734391/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2695840/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:24662/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:3243696/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:385301/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:356281/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:351279/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:491581/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:3298145/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:557865/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:643303/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:3286867/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:750454/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:3243321/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:3240599/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:654908/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:3237481/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:3204576/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:68513/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:3130444/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:386632/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1509391/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1923892/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1904941/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1821471/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1767340/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1665433/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1649666/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1630885/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1605640/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:16017/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1929485/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1501576/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1377532/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1336201/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1278041/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1261301/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1163526/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1155033/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1121120/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1038875/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:219745/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2588565/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2577167/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2573917/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2538814/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2484770/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1003806/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:246493/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2418199/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2405946/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2598162/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2159982/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:213131/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2050766/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2032559/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2029434/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2027960/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2007779/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:2006437/69‑1 (MQ=255)                                                      tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATc  <  1:1980860/69‑1 (MQ=255)                                                          |                                                                 GAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATC  >  minE/1643803‑1643924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑