Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,700,462	A→G	L24P (CTA→CCA)	hcaT ←	predicted 3‑phenylpropionic transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,700,462	0	A	G	100.0%	126.7 / NA	35	L24P (CTA→CCA)	hcaT	predicted 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/35);  total (0/35)

CGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAA  >  minE/1700448‑1700527               |                                                                  cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:1647864/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:287004/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:2721897/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:2558375/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:2211600/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:2908181/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:2105036/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:1952267/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:2990547/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:1791604/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:1791344/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:1646013/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:1584898/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:1555144/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:669906/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:1460313/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:1391012/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:805567/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:1322428/71‑1 (MQ=255) cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg           <  1:867015/71‑1 (MQ=255)      cAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGAt        <  1:1042221/69‑1 (MQ=255)      cAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGAt        <  1:3096458/69‑1 (MQ=255)      cAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGAt        <  1:2173848/69‑1 (MQ=255)      cAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGAt        <  1:671233/69‑1 (MQ=255)      cAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGAt        <  1:743414/69‑1 (MQ=255)         aaaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGAGCCAACCAGCGCGTg           <  1:603928/63‑1 (MQ=255)          aaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaaa  <  1:948210/71‑1 (MQ=255)          aaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaaa  <  1:561996/71‑1 (MQ=255)          aaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaaa  <  1:446486/71‑1 (MQ=255)          aaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaaa  <  1:3017931/71‑1 (MQ=255)          aaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaaa  <  1:1861212/71‑1 (MQ=255)          aaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaaa  <  1:1546662/71‑1 (MQ=255)          aaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaaa  <  1:1382882/71‑1 (MQ=255)          aaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaaa  <  1:1216458/71‑1 (MQ=255)          aaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaaa  <  1:1135320/71‑1 (MQ=255)               |                                                                  CGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAA  >  minE/1700448‑1700527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑