BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	195,616	C→T	L71L (CTG→CTA)	yaeF ←	predicted lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	195,616	0	C	T	100.0%	458.2 / NA	130	L71L (CTG→CTA)	yaeF	predicted lipoprotein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (95/35); total (95/35)

Only 100 of 130 total aligned reads displayed.

ACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGA  >  minE/195548‑195674

aCGTGACTCACAGAGGAAGTGCTGAAGCCGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:1021370/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:2967239/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:184225/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:204759/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:39119/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:1600560/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:698550/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:2363865/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:1485261/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:1481463/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:3272479/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:786941/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:2793361/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:1032039/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:3043597/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:1099955/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:1227853/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                          >  1:118385/1‑71 (MQ=255)

aCGTGACTCACAGAGGAAGTGCTGAAGA‑‑CGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGc                                                         >  1:440692/1‑70 (MQ=255)

  gTGACTCACAGAGGAAGTGCTGAAGTCGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGc                                                         >  1:2259833/1‑70 (MQ=255)

  gTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGc                                                         >  1:2781067/1‑70 (MQ=255)

  gTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGc                                                         >  1:1621020/1‑70 (MQ=255)

              ggAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCGAGGCTTGAGGAGAATAGCAAATCGCCGGGtt                                            >  1:832379/1‑71 (MQ=255)

              ggAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGtt                                            >  1:2876311/1‑71 (MQ=255)

              ggAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGtt                                            >  1:1616180/1‑71 (MQ=255)

              ggAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGtt                                            >  1:250147/1‑71 (MQ=255)

              ggAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGtt                                            >  1:1098542/1‑71 (MQ=255)

              ggAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGtt                                            >  1:1433018/1‑71 (MQ=255)

              ggAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGtt                                            >  1:1746581/1‑71 (MQ=255)

              ggAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGtt                                            >  1:21361/1‑71 (MQ=255)

              ggAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGtt                                            >  1:253377/1‑71 (MQ=255)

              ggAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGtt                                            >  1:1912784/1‑71 (MQ=255)

                    gCTGAAGACGCGGATTCCAAATGAGTTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt                                           >  1:1947938/1‑66 (MQ=255)

                    gCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt                                           >  1:2913959/1‑66 (MQ=255)

                    gCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt                                           >  1:1020380/1‑66 (MQ=255)

                    gCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt                                           >  1:1964250/1‑66 (MQ=255)

                    gCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGttt                                           >  1:124882/1‑66 (MQ=255)

                          gccgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGt                                 >  1:1209018/3‑70 (MQ=255)

                          gccgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:791223/3‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCATGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:830357/1‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATGGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:2349197/1‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAgg                                  >  1:2354196/1‑69 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:2293692/1‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:2549042/1‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:3111500/1‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:820334/1‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:1466322/1‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:353854/1‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:101242/1‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:1542531/1‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:446837/1‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:1851844/1‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTg                                >  1:490954/1‑71 (MQ=255)

                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCGTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGt                                 >  1:1328204/1‑70 (MQ=255)

                            cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                              <  1:1878534/71‑1 (MQ=255)

                            cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                              <  1:2822404/71‑1 (MQ=255)

                            cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                              <  1:3020601/71‑1 (MQ=255)

                            cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                              <  1:3201843/71‑1 (MQ=255)

                            cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                              <  1:751601/71‑1 (MQ=255)

                            cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                              <  1:1693852/71‑1 (MQ=255)

                            cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                              <  1:1718931/71‑1 (MQ=255)

                            cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                              <  1:482113/71‑1 (MQ=255)

                             gcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                              <  1:1787765/70‑1 (MQ=255)

                              cgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGcc                              <  1:1784419/69‑1 (MQ=255)

                                gATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTa                          <  1:777200/71‑1 (MQ=255)

                                gATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTa                          <  1:2437034/71‑1 (MQ=255)

                                gATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTa                          <  1:1455598/71‑1 (MQ=255)

                                gATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTa                          <  1:2481381/71‑1 (MQ=255)

                                gATTCCAAATGAAGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTa                          <  1:435424/71‑1 (MQ=255)

                                 aTTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTa                          <  1:1882261/70‑1 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGGAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:2837078/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:2124907/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:1129864/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:1339310/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:1398173/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:783280/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:1495885/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:1502591/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:594631/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:2067594/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:2221556/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:3041659/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:2805088/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:2694070/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:3295172/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:3246319/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:3131314/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattga             >  1:435092/1‑71 (MQ=255)

                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattg              >  1:387225/1‑70 (MQ=255)

                                                  cccAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGtt        >  1:934178/1‑71 (MQ=255)

                                                        gCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATTAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGa  <  1:318915/71‑1 (MQ=255)

                                                        gCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGa  <  1:2491072/71‑1 (MQ=255)

                                                        gCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGa  <  1:1427591/71‑1 (MQ=255)

                                                        gCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGa  <  1:2466196/71‑1 (MQ=255)

                                                        gCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGa  <  1:483295/71‑1 (MQ=255)

                                                        gCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGa  <  1:1251446/71‑1 (MQ=255)

                                                        gCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGa  <  1:2938010/71‑1 (MQ=255)

                                                        gCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGa  <  1:2144726/71‑1 (MQ=255)

                                                        gCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGa  <  1:93785/71‑1 (MQ=255)

                                                        gCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGa  <  1:964845/71‑1 (MQ=255)

ACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGA  >  minE/195548‑195674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`