Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,777,713	T→C	N105D (AAT→GAT)	trmD ←	tRNA (guanine‑1‑)‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,777,713	0	T	C	100.0%	187.1 / NA	51	N105D (AAT→GAT)	trmD	tRNA (guanine‑1‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (0/51);  total (0/51)

ATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAT  >  minE/1777658‑1777773                                                        |                                                             aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:905817/71‑1 (MQ=255) aTCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                               <  1:1790/71‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:2572841/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:2624361/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:2259346/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:2235492/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:2698685/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:2195679/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:2193506/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:2180061/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:273497/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:2748424/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:1937395/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:1935755/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:2920292/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:1676830/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:3045183/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:1436470/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:430045/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:637794/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:726572/69‑1 (MQ=255)            aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCCTTGCCAGTTCGCTGACGCCCGc                                      <  1:2910939/69‑1 (MQ=255)             tCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:2720449/68‑1 (MQ=255)             tCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:936437/68‑1 (MQ=255)                                  gacagCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                      <  1:2138846/46‑1 (MQ=38)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCGCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:1550316/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:304799/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:1056139/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:926531/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:134060/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:900155/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:893514/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:811658/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:776894/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:1360027/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:650483/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:137539/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:1433476/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:359858/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:333115/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:2527258/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:2985550/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:1679514/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:2016996/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:2882738/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:2830680/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:2176397/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:2180787/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:2210069/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:2435926/71‑1 (MQ=255)                                              aaTTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt  <  1:2446036/71‑1 (MQ=255)                                                        |                                                             ATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAT  >  minE/1777658‑1777773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑