Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,784,184 T→C I223T (ATA→ACA)  yfjB → NAD kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,784,1840TC100.0% 403.6 / NA 107I223T (ATA→ACA) yfjBNAD kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (61/46);  total (61/46)

Only 100 of 107 total aligned reads displayed.

TTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGC  >  minE/1784162‑1784248
                      |                                                                
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCt                                          >  1:2421190/1‑47 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTg                                         >  1:1127848/1‑48 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAAc                    >  1:1393009/1‑69 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:516003/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:2280162/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:2339873/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:2384410/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:2531328/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:2535932/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:2638112/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:2859099/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:2865670/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:2979740/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:305670/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:438235/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:2544590/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:525268/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:576755/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:657015/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:787887/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:788247/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:811194/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:81641/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:835504/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:958917/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:982737/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:145853/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:1693701/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:1339156/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:17599/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:1199476/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:1774835/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:1081582/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:1430158/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                  >  1:1040563/1‑71 (MQ=255)
ttGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGGTTTTCGCATCGCCGTAACGa                  >  1:1712711/1‑71 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:2520610/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:1592376/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:560870/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:2746394/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:268134/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:2664856/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:649468/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:651233/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:1925935/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:1526719/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:1738998/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:2505009/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:739253/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:3123520/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:3129495/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:2141805/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:3184634/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:922588/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:2168307/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:104716/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:2171642/70‑1 (MQ=255)
  gTCAGCACGACCACTGGTCACAAACACCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:3214662/70‑1 (MQ=255)
             cACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                 <  1:1905309/59‑1 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                              >  1:891254/1‑43 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:506021/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:1459232/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:392026/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:684295/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:621022/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:647462/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:3028536/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:750364/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:78070/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:1163617/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:899692/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:1035120/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:3215442/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:2215668/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:182543/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:2385572/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:2484362/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:2600379/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:1700078/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:2812326/1‑44 (MQ=255)
                tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                             >  1:1567891/1‑44 (MQ=255)
                  gTCACAAACAGCAGCAGCACTATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:3049165/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGTCCTGGAAATCAGTTGc  <  1:1767539/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:3040292/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:1914206/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:2129068/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:2167932/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:349348/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:2218204/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:877247/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:2224136/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:2877879/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:1311404/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:2386957/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:2439187/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:25438/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:1003328/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:2960828/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:2898281/69‑1 (MQ=255)
                  gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc  <  1:1405146/69‑1 (MQ=255)
                      |                                                                
TTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGC  >  minE/1784162‑1784248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑