Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,833,531	C→T	R92C (CGC→TGC)	ygbL →	predicted class II aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,833,531	0	C	T	100.0%	299.6 / NA	90	R92C (CGC→TGC)	ygbL	predicted class II aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (56/34);  total (56/34)

GTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCT  >  minE/1833495‑1833595                                     |                                                                 gTGCTCTTTCATCTGGCGCTGTTTCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:215218/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg                                                    >  1:2058184/1‑51 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTTGTGCATTTGCACAGCACATg                                <  1:2479285/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcacagcac                                   >  1:1096451/1‑68 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTTCACAGCACATg                                >  1:870989/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:3101928/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:2362712/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:2973913/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:2966242/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:2865297/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:2815457/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:2795381/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:2668976/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:265696/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:2578628/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:2557871/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:2543873/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:2531215/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:999924/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:431301/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:1006568/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:940988/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:890009/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:853730/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:647782/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:635445/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:632523/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:580958/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:3117216/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:426051/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:414753/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:400209/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:380930/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:37834/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:342296/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:327315/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:1983679/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:1708995/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:1841890/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:1912306/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:1536698/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:1439642/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:1396162/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:139469/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:2000546/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:1386298/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:2063236/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:1357290/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:1352841/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:2243915/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:1225593/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:1195377/71‑1 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:2294447/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                >  1:1187695/1‑71 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGCTGGTGCATTTGCACAGCACAt                                 >  1:882003/1‑70 (MQ=255) gTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGCAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:368948/71‑1 (MQ=255)  tGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:1975495/70‑1 (MQ=255)  tGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:1940777/70‑1 (MQ=255)  tGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:1622418/70‑1 (MQ=255)         tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGc                                                        >  1:1732441/1‑39 (MQ=255)         tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACgg                        >  1:1773580/1‑71 (MQ=255)         tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACgg                        >  1:900445/1‑71 (MQ=255)         tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACgg                        >  1:3087667/1‑71 (MQ=255)         tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACgg                        >  1:817977/1‑71 (MQ=255)         tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACgg                        >  1:1675964/1‑71 (MQ=255)         tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACgg                        >  1:2934985/1‑71 (MQ=255)         tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACgg                        >  1:14676/1‑71 (MQ=255)         tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACgg                        >  1:439060/1‑71 (MQ=255)         tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACgg                        >  1:19804/1‑71 (MQ=255)                gcgcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCttt                  <  1:232890/70‑1 (MQ=255)                gcgcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCttt                  <  1:2210236/70‑1 (MQ=255)                gcgcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCttt                  <  1:2612149/70‑1 (MQ=255)                gcgcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCttt                  <  1:2742929/70‑1 (MQ=255)                gcgcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCttt                  <  1:599766/70‑1 (MQ=255)                gcgcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCttt                  <  1:2827849/70‑1 (MQ=255)                gcgcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCttt                  <  1:2965922/70‑1 (MQ=255)                gcgcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCAATTGCACAGCACATGGTCGACGGCGCttt                  <  1:1701097/70‑1 (MQ=255)                gcgcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGGGCATTTGCACAGCACATGGTCGACGGCGCttt                  <  1:2441074/70‑1 (MQ=255)                   cTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCttt                  <  1:893700/67‑1 (MQ=255)                           caacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATg                                <  1:209939/45‑1 (MQ=255)                               aaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGCCGGCGCTTTCCTGCCTGCAAGGGCt  >  1:2390768/1‑71 (MQ=255)                               aaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCt  >  1:514007/1‑71 (MQ=255)                               aaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCt  >  1:648848/1‑71 (MQ=255)                               aaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCt  >  1:696960/1‑71 (MQ=255)                               aaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCt  >  1:3035861/1‑71 (MQ=255)                               aaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCt  >  1:2149551/1‑71 (MQ=255)                               aaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCt  >  1:1301654/1‑71 (MQ=255)                               aaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCt  >  1:2306914/1‑71 (MQ=255)                               aaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCt  >  1:3173740/1‑71 (MQ=255)                               aaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCt  >  1:1779329/1‑71 (MQ=255)                                     |                                                                 GTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCT  >  minE/1833495‑1833595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑